prikaz prve stranice dokumenta Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
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Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
Bantao Journal, 18 (2020), 1; 24-30. urn:nbn:hr:184:001617
Katalinić, Nataša; Crnić Marčetić, Tajana; Mavrinac, Martina; Starčević, Alma; Balen, Anita; Balen, Sanja

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Katalinić, N., Crnić Marčetić, T., Mavrinac, M., Starčević, A., Balen, A. i Balen, S. (2020). Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen. Bantao Journal, 18. (1), 24-30. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, Nataša, et al. "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen." Bantao Journal, vol. 18, br. 1, 2020, str. 24-30. https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, Nataša, Tajana Crnić Marčetić, Martina Mavrinac, Alma Starčević, Anita Balen i Sanja Balen. "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen." Bantao Journal 18, br. 1 (2020): 24-30. https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, N., et al. (2020) 'Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen', Bantao Journal, 18(1), str. 24-30. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:001617 (Datum pristupa: 19.11.2024.)

Katalinić N, Crnić Marčetić T, Mavrinac M, Starčević A, Balen A, Balen S. Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen. Bantao Journal [Internet]. 2020. [pristupljeno 19.11.2024.];18(1):24-30. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:001617

N. Katalinić, T. Crnić Marčetić, M. Mavrinac, A. Starčević, A. Balen i S. Balen, "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen", Bantao Journal, vol. 18, br. 1, str. 24-30, 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:001617. [Citirano: 19.11.2024.]

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Naslov (engleski)Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
AutorNataša Katalinić
AutorTajana Crnić Marčetić
AutorMartina Mavrinac
AutorAlma Starčević
AutorAnita Balen
AutorSanja Balen
MBZ: 236875
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za kliničko-laboratorijsku dijagnostiku)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Introduction. Antibodies to human leukocyte antigens (HLA) are one of the major immune barriers to successful organ transplantation. In addition to complementdependent cytotoxicity (CDC) assay as a standard method for the HLA antibodies detection, more sensitive solid-phase assays like Luminex were introduced. The aim of this study was to define mean fluorescence intensity (MFI) cutoff values of HLA Class I antibodies detected by Luminex from those detected by CDC given the possibility to use only Luminex assay in HLA antibody screening. Methods. This is a retrospective analysis of the HLA antibody screening results of patients on a kidney transplant waiting list, performed at the Tissue Typing Laboratory, Clinical Hospital Center Rijeka, Croatia, from January 2012 to July 2019. The study included 1,665 sera tested in parallel by CDC and Luminex techniques. Results. Almost half sera contained HLA antibodies (47.9%), significantly more detected by the Luminex than a CDC method. Antibodies against HLA-A and HLA-B molecules had higher MFI values, relative to the HLA-C antibodies, as well as antibodies detected by Luminex and CDC than those detected by Luminex alone. A cutoff MFI≥9,204 for Luminex detected HLA Class I antibodies correlated with positivity in the CDC assay. Besides MFI, several factors need to be taken into consideration in interpreting test results to identify unacceptable antigen mismatch. Conclusion. The results of this analysis suggest that the current features of the Luminex technique provide the most benefit in the HLA antibody scre.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBantao Journal
Brojčani podacivol. 18, br. 1, str. 24-30
e-ISSN1312-2517
URN:NBNurn:nbn:hr:184:001617
Datum objave publikacije2020
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-03-03 19:25:38