Introduction: Dentine hypersensitivity is a common finding, and it followed by severe and sharp pain on physical or chemical stimulation, which significantly affects the patients' daily life and, subsequently, impairs their quality of life. Objective: To determine the association between dentine hypersensitivity and initial periodontal therapy. Materials and methods: 25 subjects included in the supportive therapy at the Clinical Hospital Center Rijeka filled out a modified version of the questionnaire "Dentine hypersensitivity experience questionnaire" (DHEQ) to assess the quality of life. The questionnaire is classified according to the Likert scale and consists of fifteen questions that assess the impact of dentinal hypersensitivity on the domain of limitation, confrontation, social interactions, emotional interactions and personal identity. Results: There were no statistically significant differences in the observed domains, although the subjects confirmed that they have the greatest difficulties in the domain of limitations and emotional interactions. The influence of age, gender, and oral hygiene on the degree of dentine hypersensitivity in all domains showed no significance. Conclusion: The duration of supportive therapy is inversely proportional to the degree of difficulty in all domains. Due to the adjustment of the subjects to dentinal hypersensitivity over a long period of supportive therapy, they felt the greatest relief in the domain of emotional interactions. Special toothpastes targeting dentinal hypersensitivity also contribute to a better quality of life despite the persistence of the condition.