Cytomegalovirus (CMV) is an important pathogen that extensively remodels the nucleus and cytosol of an infected cell to establish a productive infection [1,2,3,4]. Nuclear events include the formation of large structures that are known as nuclear replication centers (NRCs), where viral DNA replication and nuclear capsid assembly occur. Cytosolic events include the complete reorganization of the cytoskeleton and the membrane system (Figure 1). The reorganized membrane system (RMS) of the infected cell involves the relocation of the Golgi into a ring-like configuration that encloses a large perinuclear region containing early endosomes (EEs), recycling endosomes (REs), the trans-Golgi network (TGN), and expanded membrane structures of membrane intermediates at the EE-RE/ERC-TGN interface (Figure 1B) [5,6,7,8]. This structure, which is as large as the nucleus of the infected cell, is referred to as the cytoplasmic assembly complex (cAC) and is likely the site of the final steps of CMV virion assembly, including the envelopment of the tegumented capsids by cellular membranes and the establishment of the pathway for virion egress from the cell [2]. The endoplasmic reticulum (ER) and late endosomes (LEs) are extruded together with the secretory system of the cell from the AC towards the cell periphery (Figure 1B) [2,5,8]. This extensive reorganization of the membrane system, which is often accompanied by a compaction of the cell due to the restructuring of the cytoskeleton, obviously involves a redirection of membrane flux that is difficult to compare with that in the flat cell. Overall, little is known about the transport pathways, membrane flux, and remodeling of membrane organelles in such a reorganized membrane system.