prikaz prve stranice dokumenta Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro
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Scientific paper - Original scientific paper
Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro
Cells, 13 (2024), 3; 225. https://doi.org/10.3390/cells13030225
Gržeta Krpan, Nika; Harej Hrkać, Anja; Janković, Tamara; Dolenec, Petra; Bekyarova, Elena; Parpura, Vladimir; Pilipović, Kristina

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Gržeta Krpan, N., Harej Hrkać, A., Janković, T., Dolenec, P., Bekyarova, E., Parpura, V. & Pilipović, K. (2024). Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro. Cells, 13. (3). doi: 10.3390/cells13030225

Gržeta Krpan, Nika, et al. "Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro." Cells, vol. 13, no. 3, 2024. https://doi.org/10.3390/cells13030225

Gržeta Krpan, Nika, Anja Harej Hrkać, Tamara Janković, Petra Dolenec, Elena Bekyarova, Vladimir Parpura and Kristina Pilipović. "Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro." Cells 13, no. 3 (2024). https://doi.org/10.3390/cells13030225

Gržeta Krpan, N., et al. (2024) 'Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro', Cells, 13(3). doi: 10.3390/cells13030225

Gržeta Krpan N, Harej Hrkać A, Janković T, Dolenec P, Bekyarova E, Parpura V, and sur.. Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro. Cells [Internet]. 2024 January 25 [cited 2024 December 07];13(3). doi: 10.3390/cells13030225

N. Gržeta Krpan, et al., "Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro", Cells, vol. 13, no. 3, January 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:201849. [Accessed: 07 December 2024]

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Title (english)Chemically Functionalized Single-Walled Carbon Nanotubes Prevent the Reduction in Plasmalemmal Glutamate Transporter EAAT1 Expression in, and Increase the Release of Selected Cytokines from, Stretch-Injured Astrocytes in Vitro
AuthorNika Gržeta Krpan
AuthorAnja Harej Hrkać
AuthorTamara Janković
AuthorPetra Dolenec
AuthorElena Bekyarova
AuthorVladimir Parpura
AuthorKristina Pilipović
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Pharmacology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract (english)
We tested the effects of water-soluble single-walled carbon nanotubes, chemically functionalized with polyethylene glycol (SWCNT-PEG), on primary mouse astrocytes exposed to a severe in vitro simulated traumatic brain injury (TBI). The application of SWCNT-PEG in the culture media of injured astrocytes did not affect cell damage levels, when compared to those obtained from injured, functionalization agent (PEG)-treated cells. Furthermore, SWCNT-PEG did not change the levels of oxidatively damaged proteins in astrocytes. However, this nanomaterial prevented the reduction in plasmalemmal glutamate transporter EAAT1 expression caused by the injury, rendering the level of EAAT1 on par with that of control, uninjured PEG-treated astrocytes; in parallel, there was no significant change in the levels of GFAP. Additionally, SWCNT-PEG increased the release of selected cytokines that are generally considered to be involved in recovery processes following injuries. As a loss of EAATs has been implicated as a culprit in the suffering of human patients from TBI, the application of SWCNT-PEG could have valuable effects at the injury site, by preventing the loss of astrocytic EAAT1 and consequently allowing for a much-needed uptake of glutamate from the extracellular space, the accumulation of which leads to unwanted excitotoxicity. Additional potential therapeutic benefits could be reaped from the fact that SWCNT-PEG stimulated the release of selected cytokines from injured astrocytes, which would promote recovery after injury and thus counteract the excess of proinflammatory cytokines present in TBI.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCells
Numberingvol. 13, no. 3, 225
e-ISSN2073-4409
DOIhttps://doi.org/10.3390/cells13030225
URN:NBNurn:nbn:hr:184:201849
Publication2024-01-25
ProjectNumber: UIP-2017-05-9517
Title (croatian): Jednozidne ugljikove nanocjevčice u eksperimentalnoj traumatskoj ozljedi mozga
Title (english): Single walled carbon nanotubes in experimental traumatic brain injury
Acronym: CarNanoTBI
Leader: Kristina Pilipović
Jurisdiction: Croatia
Funding stream: UIP
Document URLhttps://www.mdpi.com/2073-4409/13/3/225
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-02-16 08:40:29