prikaz prve stranice dokumenta Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both
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Scientific paper - Original scientific paper
Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both
Scientific Reports, 13 (2023), 1; 21390. https://doi.org/10.1038/s41598-023-48397-7
Beitari, Saina; Agbayani, Gerard; Hewitt, Melissa; Duque, Diana; Bavananthasivam, Jegarubee; Sandhu, Jagdeep K.; Akache, Bassel; Hadžisejdić, Ita; Tran, Anh

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Beitari, S., Agbayani, G., Hewitt, M., Duque, D., Bavananthasivam, J., Sandhu, J. K. ... Tran, A. (2023). Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both. Scientific Reports, 13. (1). doi: 10.1038/s41598-023-48397-7

Beitari, Saina, et al. "Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both." Scientific Reports, vol. 13, no. 1, 2023. https://doi.org/10.1038/s41598-023-48397-7

Beitari, Saina, Gerard Agbayani, Melissa Hewitt, Diana Duque, Jegarubee Bavananthasivam, Jagdeep K. Sandhu, Bassel Akache, Ita Hadžisejdić and Anh Tran. "Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both." Scientific Reports 13, no. 1 (2023). https://doi.org/10.1038/s41598-023-48397-7

Beitari, S., et al. (2023) 'Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both', Scientific Reports, 13(1). doi: 10.1038/s41598-023-48397-7

Beitari S, Agbayani G, Hewitt M, Duque D, Bavananthasivam J, Sandhu JK, and sur.. Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both. Scientific Reports [Internet]. 2023 December 04 [cited 2025 January 23];13(1). doi: 10.1038/s41598-023-48397-7

S. Beitari, et al., "Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both", Scientific Reports, vol. 13, no. 1, December 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:514775. [Accessed: 23 January 2025]

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Metadata
Title (english)Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both
AuthorSaina Beitari
AuthorGerard Agbayani
AuthorMelissa Hewitt
AuthorDiana Duque
AuthorJegarubee Bavananthasivam
AuthorJagdeep K. Sandhu
AuthorBassel Akache
AuthorIta Hadžisejdić
AuthorAnh Tran
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pathology
Abstract (english)
Abstract A critical feature of the VSV vector platform is the ability to pseudotype the virus with different glycoproteins from other viruses, thus altering cellular tropism of the recombinant virus. The route of administration is critical in triggering local and systemic immune response and protection. Most of the vaccine platforms used at the forefront are administered by intramuscular injection. However, it is not known at what level ACE2 is expressed on the surface of skeletal muscle cells, which will have a significant impact on the efficiency of a VSV-SARS-CoV-2 spike vaccine to mount a protective immune response when administered intramuscularly. In this study, we investigate the immunogenicity and efficacy of a prime-boost immunization regimen administered intranasally (IN), intramuscularly (IM), or combinations of the two. We determined that the prime-boost combinations of IM followed by IN immunization (IM + IN) or IN followed by IN immunization (IN + IN) exhibited strong spike-specific IgG, IgA and T cell response in vaccinated K18 knock-in mice. Hamsters vaccinated with two doses of VSV expressing SARS-CoV-2 spike, both delivered by IN or IM + IN, showed strong protection against SARS-CoV-2 variants of concern Alpha and Delta. This protection was also observed in aged hamsters. Our study underscores the highly crucial role immunization routes have with the VSV vector platform to elicit a strong and protective immune response.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 13, no. 1, 21390
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-023-48397-7
URN:NBNurn:nbn:hr:184:514775
Publication2023-12-04
Document URLhttps://www.nature.com/articles/s41598-023-48397-7
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-12-19 08:49:12