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Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions
At: 46th International Herpesvirus Workshop. Virtual meeting, United States, 2022. urn:nbn:hr:184:244380
Marcelić, Marina; Mahmutefendić Lučin, Hana; Jurak Begonja, Antonija; Blagojević Zagorac, Gordana; Viduka, Ivona; Lučin, Pero

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Marcelić, M., Mahmutefendić Lučin, H., Jurak Begonja, A., Blagojević Zagorac, G., Viduka, I. & Lučin, P. Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, Marina, et al. "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions." 46th International Herpesvirus Workshop, 25.07.2022.-29.07.2022., Virtual meeting, United States. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, Marina, Hana Mahmutefendić Lučin, Antonija Jurak Begonja, Gordana Blagojević Zagorac, Ivona Viduka and Pero Lučin. "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions." Presentation at 46th International Herpesvirus Workshop, Virtual meeting, United States, 25.07.2022.-29.07.2022. https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, M., et al. 'Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions' [Presentation], 46th International Herpesvirus Workshop. 25.07.2022.-29.07.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić M, Mahmutefendić Lučin H, Jurak Begonja A, Blagojević Zagorac G, Viduka I, Lučin P. Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions. [Presentation] [cited 2024 November 12] Available at: https://urn.nsk.hr/urn:nbn:hr:184:244380

M. Marcelić, H. Mahmutefendić Lučin, A. Jurak Begonja, G. Blagojević Zagorac, I. Viduka and P. Lučin, "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions", presented at: 46th International Herpesvirus Workshop, Virtual meeting, 25.07.2022.-29.07.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:244380

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Title (english)Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions
AuthorMarina Marcelić
AuthorHana Mahmutefendić Lučin
AuthorAntonija Jurak Begonja
AuthorGordana Blagojević Zagorac
AuthorIvona Viduka
AuthorPero Lučin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Cytomegaloviruses (CMVs) initiate the stepwise establishment of their cytoplasmic virion assembly compartment (cVAC) at the earliest stages of infection by expanding the interface between early endosomes (EEs) and endosomal recycling compartment (ERC) and relocating the Golgi complex. This membranous organelle reorganization involves phosphatidylinositol-3-phosphate (PI(3)P)-containing membrane domains, as PI(3)P is the master regulator of the EE pathway. Our goal was to investigate the contribution of PI(3)P-dependent EE domains to cVAC biogenesis and their role in the consequent progression of the CMV life cycle. Our functional analysis of PI(3)P was based on rapid depletion of PI(3)P production at EE membranes using VPS34-IN1, a specific and reversible inhibitor of Vps34 component of class III PI(3)P kinase, and on saturation of PI(3)P by overexpression of recombinant PI(3)P-binding domains (2xFYVE, p40PX). PI(3)P depletion led to rapid reorganization of the Rab11a-dependent pathway of the ERC, and abolished recycling cargo sorting in this pathway as well as membrane flux from EE to the ERC. Despite these alterations and although PI(3)P+ and Vps34+ endosomes build a substantial part of cVAC, the PI(3)P inhibition did not prevent the establishment of infection, rogression through the early phase of infection, and membrane reorganization associated with cVAC development, as analyzed by confocal imaging and Western blot analysis. However, the drastically reduced viral growth under PI(3)P depletion and the significant inhibition of late infection events (DNA replication, late protein expression) together with the reduced release of infectious virions suggest that PI(3)P is essential for entry into the late phase of infection and the final formation of progeny virions.
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:244380
Presentation date2022-07-26
ProjectNumber: IP-2019-04-3582
Title (croatian): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Leader: Pero Lučin
Jurisdiction: Croatia
ProjectNumber: IP-2020-02-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Leader: Gordana Blagojević Zagorac
Jurisdiction: Croatia
ProjectNumber: IP-2020-02-2916
Title (croatian): Reorganizacija sučelja između ranih endosoma i endosomalnog reciklirajućeg odjeljka
Leader: Hana Mahmutefendić Lučin
Jurisdiction: Croatia
ConferenceTitle: 46th International Herpesvirus Workshop
Location: Virtual meeting, United States
Start date: 2022-07-25
End date: 2022-07-29
Assembly type: Lecture
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-04-05 11:44:12