prikaz prve stranice dokumenta Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection
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Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection
At: The 45th FEBS Virtual Congress. Virtual meeting, Slovenia, 2021. urn:nbn:hr:184:007576
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lučin, Pero; Blagojević Zagorac, Gordana

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Pavišić, V., Mahmutefendić Lučin, H., Lučin, P. & Blagojević Zagorac, G. Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:007576

Pavišić, Valentino, et al. "Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection." The 45th FEBS Virtual Congress, 03.07.2021.-08.07.2021., Virtual meeting, Slovenia. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:007576

Pavišić, Valentino, Hana Mahmutefendić Lučin, Pero Lučin and Gordana Blagojević Zagorac. "Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection." Presentation at The 45th FEBS Virtual Congress, Virtual meeting, Slovenia, 03.07.2021.-08.07.2021. https://urn.nsk.hr/urn:nbn:hr:184:007576

Pavišić, V., et al. 'Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection' [Presentation], The 45th FEBS Virtual Congress. 03.07.2021.-08.07.2021. Available at: https://urn.nsk.hr/urn:nbn:hr:184:007576

Pavišić V, Mahmutefendić Lučin H, Lučin P, Blagojević Zagorac G. Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection. [Presentation] [cited 2024 November 12] Available at: https://urn.nsk.hr/urn:nbn:hr:184:007576

V. Pavišić, H. Mahmutefendić Lučin, P. Lučin and G. Blagojević Zagorac, "Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection", presented at: The 45th FEBS Virtual Congress, Virtual meeting, 03.07.2021.-08.07.2021. Available at: https://urn.nsk.hr/urn:nbn:hr:184:007576

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Metadata
Title (english)Arf proteins accumulate in the citomegalovirus assembly compartment during the early phase of infection
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorPero Lučin
AuthorGordana Blagojević Zagorac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Citomegaloviruses (CMVs) are DNA viruses that can cause severe disease in immunocompromised persons. Very early during infection, CMVs extensively reorganize membranous compartments of the host cells which finally results with formation of virion assembly compartment (VAC). Since Arf GTPases are one of the major regulators of cellular membrane dynamics we hypothesized that CMVs manipulate Arf protein system in order to ensure favorable conditions for their replication. To address this hypothesis, we infected Balb 3T3 cells with murine CMV (MCMV), since organization structure of human and murine VAC is similar but at the same time murine MCMV replication cycle is much shorter and therefore more suitable for studying. In MCMV infected cells expression of class I (Arf1, Arf3), clas II (Arf4, Arf5) and clas II (Arf6) proteins was determined with Western blot and immunofluorescent microscopy in the early phase of the infection. To determine their intracellular localization, each of the Arf proteins was colocalized with markers of early and late endosomal compartments, markers of Golgi complex, as well as with different early phase viral proteins. Our results indicate that Arf proteins are overexpressed (Arf1-5) or overactivated (Arf6) during the early phase of CMV infection. They also change their intracellular localization and are accumulated in the inner part of VAC where they colocalize with markers of early and recycling endosomes. Better understanding of VAC formation and the role that Arf proteins play in that process may enable development of new antiviral and therapeutic strategies.
Keywords (english)
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:007576
Presentation date2021-07-06
ConferenceTitle: The 45th FEBS Virtual Congress
Acronym: FEBS
Location: Virtual meeting, Slovenia
Start date: 2021-07-03
End date: 2021-07-08
Assembly type: Lecture
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2023-04-05 07:47:38