prikaz prve stranice dokumenta Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
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Scientific paper - Original scientific paper
Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
Antimicrobial Agents and Chemotherapy, 41 (1997), 12; 2686-2692. urn:nbn:hr:184:610927
Pavić, Ivica; Hartmann, Anke; Zimmerman, Albert; Michel, Detlef; Hampl, Walter; Schleyer, Ingrid; Mertens, Thomas

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Pavić, I., Hartmann, A., Zimmerman, A., Michel, D., Hampl, W., Schleyer, I. & Mertens, T. (1997). Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet. Antimicrobial Agents and Chemotherapy, 41. (12), 2686-2692. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, Ivica, et al. "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet." Antimicrobial Agents and Chemotherapy, vol. 41, no. 12, 1997, pp. 2686-2692. https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, Ivica, Anke Hartmann, Albert Zimmerman, Detlef Michel, Walter Hampl, Ingrid Schleyer and Thomas Mertens. "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet." Antimicrobial Agents and Chemotherapy 41, no. 12 (1997): 2686-2692. https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, I., et al. (1997) 'Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet', Antimicrobial Agents and Chemotherapy, 41(12), pp. 2686-2692. Available at: https://urn.nsk.hr/urn:nbn:hr:184:610927 (Accessed 15 November 2024)

Pavić I, Hartmann A, Zimmerman A, Michel D, Hampl W, Schleyer I, and sur.. Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet. Antimicrobial Agents and Chemotherapy [Internet]. 1997 [cited 2024 November 15];41(12):2686-2692. Available at: https://urn.nsk.hr/urn:nbn:hr:184:610927

I. Pavić, et al., "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet", Antimicrobial Agents and Chemotherapy, vol. 41, no. 12, pp. 2686-2692, 1997. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:610927. [Accessed: 15 November 2024]

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Title (english)Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
AuthorIvica Pavić
AuthorAnke Hartmann
AuthorAlbert Zimmerman
AuthorDetlef Michel
AuthorWalter Hampl
AuthorIngrid Schleyer
AuthorThomas Mertens
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Infectious Diseases)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Infectology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
We established a quantitative flow cytometric method for determination of herpes simplex virus type 1 (HSV-1) susceptibility to acyclovir (ACV), ganciclovir, and foscarnet in vitro. Susceptibility was defined in terms of the drug concentration which reduced the number of cells expressing HSV-1 glycoprotein C (gpC) with a fluorescence intensity of > or =10(2) by 50% (IC50). Flow cytometry allowed us to use a high (1.0) as well as a low (0.005) multiplicity of infection, and determination of the IC50 was possible after one or more viral replicative cycles. IC50s were dependent on virus input and on time postinfection. In mixture experiments, 1 to 2% resistant viruses added to a sensitive strain could be detected. The results obtained by flow cytometry showed a good qualitative correlation with those achieved by cytopathic effect inhibitory assay. However, flow cytometry might detect more quantitative differences in drug susceptibility, especially among resistant strains, as confirmed also by determination of intracellular drug phosphorylation. The mean IC50s for ACV-sensitive strains were 0.45 to 1.47 microM, and those for ACV-resistant strains were between 140 and 3, 134 microM. Flow cytometric analysis was fast and accurate, automatizable, and highly reproducible. Flow cytometry may be a more powerful tool than standard cytopathic effect-based assays and could have advantages for the detection of low levels of drug resistance or mixtures of sensitive and resistant virus strains.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleAntimicrobial Agents and Chemotherapy
Numberingvol. 41, no. 12, pp. 2686-2692
p-ISSN0066-4804
URN:NBNurn:nbn:hr:184:610927
Publication1997
Document URLhttp://bib.irb.hr/811830
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-04-20 14:01:53