prikaz prve stranice dokumenta Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis
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Scientific paper - Original scientific paper
Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis
Journal of rheumatology, 30 (2003), 4; 660-670. urn:nbn:hr:184:356091
Gulan, Gordan; Ravlić-Gulan, Jagoda; Štrbo, Nataša; Sotošek, Vlatka; Nemec, Boris; Matovinović, Damir; Rubinić, Dušan; Podack, Eckhard R.; Rukavina, Daniel

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Gulan, G., Ravlić Gulan, J., Štrbo, N., Sotošek, V., Nemec, B., Matovinović, D. ... Rukavina, D. (2003). Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis. Journal of rheumatology, 30. (4), 660-670. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:356091

Gulan, Gordan, et al. "Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis." Journal of rheumatology, vol. 30, no. 4, 2003, pp. 660-670. https://urn.nsk.hr/urn:nbn:hr:184:356091

Gulan, Gordan, Jagoda Ravlić Gulan, Nataša Štrbo, Vlatka Sotošek, Boris Nemec, Damir Matovinović, Dušan Rubinić, Eckhard R. Podack and Daniel Rukavina. "Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis." Journal of rheumatology 30, no. 4 (2003): 660-670. https://urn.nsk.hr/urn:nbn:hr:184:356091

Gulan, G., et al. (2003) 'Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis', Journal of rheumatology, 30(4), pp. 660-670. Available at: https://urn.nsk.hr/urn:nbn:hr:184:356091 (Accessed 24 April 2025)

Gulan G, Ravlić Gulan J, Štrbo N, Sotošek V, Nemec B, Matovinović D, and sur.. Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis. Journal of rheumatology [Internet]. 2003 [cited 2025 April 24];30(4):660-670. Available at: https://urn.nsk.hr/urn:nbn:hr:184:356091

G. Gulan, et al., "Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis", Journal of rheumatology, vol. 30, no. 4, pp. 660-670, 2003. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:356091. [Accessed: 24 April 2025]

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Metadata
Title (english)Systemic and local expression of perforin in lymphocyte subsets in acute and chronic rheumatoid arthritis
AuthorGordan Gulan
AuthorJagoda Ravlić-Gulan
AuthorNataša Štrbo
AuthorVlatka Sotošek
AuthorBoris Nemec
AuthorDamir Matovinović
AuthorDušan Rubinić
AuthorEckhard R. Podack
AuthorDaniel Rukavina
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Orthopedics and Physical Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Many different models exist that try to explain the pathogenesis of rheumatoid arthritis. However the perpetuation of synovitis in rheumatoid arthritis likely results from a complicated interplay among lymphocytes, fibroblast-like cells and macrophage-like cells. Unraveling and understanding the highly complex inter-relationship between these cells causing the initiation and perpetuation of rheumatoid arthritis would be very helpful in finding successful therapeutic approaches. In this study we... More analysed the potential pathophysiologic role of perforin-positive cells in the destructive proccesses of rheumatoid arthritis. These cells, alone or in combination with other approaches, could offer a novel therapeutic target for interfering with the chronic inflammatory proccesses. Objective: To obtain insight into the potential role of the cytolytic action mediated by perforin in the course of rheumatoid arthritis, we studied the immunophenotypic characteristics of lymphocytes containing perforin simultaneously in peripheral blood (systemic level) and in synovial fluid and synovial membrane (local level) in patients in acute or chronic phase of rheumatoid arthritis. As controls served cells from patients suffering of osteoarthrosis. Methods: Our procedure for simultaneous detection of intracellular (perforin) and cell surface antigens by flow cytometry was used. Mean fluorescence intensity (MFI) was a measure of the mean perforin content per cell. To determine the localization of perforin positive cells immunohistochemical staining methods of frozen sections were used. Results: Significantly higher percentages of perforin positive cells as well as relative numbers of perforin molecules per cell, were found at both the systemic and local level in the acute phase of rheumatoid arthritis in comparison with other groups. Perforin expression in acute rheumatoid arthritis was higher at the systemic level (peripheral blood) in comparison with local level (synovial membrane and synovial fluid), but the relative increase of perforin positive cells was much higher at the local level. Conclusions: Perforin positive cells participate in the acute phase of rheumatoid arthritis, maintaining and perpetuating the immunological events, and probably contributing to the tissue destruction. Less
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of rheumatology
Numberingvol. 30, no. 4, pp. 660-670
p-ISSN0315-162X
URN:NBNurn:nbn:hr:184:356091
Publication2003
Document URLhttp://bib.irb.hr/95502
Type of resourceText
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2022-03-30 16:14:53
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