prikaz prve stranice dokumenta Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids
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Scientific paper - Original scientific paper
Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids
Current Vascular Pharmacology, 17 (2019), 5; 507-516. https://doi.org/10.2174/1570161117666190705141152
M’baya-Moutoula, Eleonore; Marchand, Alexandre; Six, Isabelle; Bahrar, Noura; Ćelić, Tanja; Mougenot, Nathalie; Maitrias, Pierre; Massy, Ziad A.; Lompreh, Anne-Marie; Metzinger, Laurent; Meuth, Valérie Metzinger-Le

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M’baya Moutoula, E., Marchand, A., Six, I., Bahrar, N., Ćelić, T., Mougenot, N. ... Meuth, V. M. L. (2019). Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids. Current Vascular Pharmacology, 17. (5), 507-516. doi: 10.2174/1570161117666190705141152

M’baya Moutoula, Eleonore, et al. "Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids." Current Vascular Pharmacology, vol. 17, no. 5, 2019, pp. 507-516. https://doi.org/10.2174/1570161117666190705141152

M’baya Moutoula, Eleonore, Alexandre Marchand, Isabelle Six, Noura Bahrar, Tanja Ćelić, Nathalie Mougenot, Pierre Maitrias, et al. "Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids." Current Vascular Pharmacology 17, no. 5 (2019): 507-516. https://doi.org/10.2174/1570161117666190705141152

M’baya Moutoula, E., et al. (2019) 'Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids', Current Vascular Pharmacology, 17(5), pp. 507-516. doi: 10.2174/1570161117666190705141152

M’baya Moutoula E, Marchand A, Six I, Bahrar N, Ćelić T, Mougenot N, and sur.. Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids. Current Vascular Pharmacology [Internet]. 2019 [cited 2024 December 26];17(5):507-516. doi: 10.2174/1570161117666190705141152

E. M’baya Moutoula, et al., "Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids", Current Vascular Pharmacology, vol. 17, no. 5, pp. 507-516, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:088359. [Accessed: 26 December 2024]

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Metadata
Title (english)Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids
AuthorEleonore M’baya-Moutoula
AuthorAlexandre Marchand
AuthorIsabelle Six
AuthorNoura Bahrar
AuthorTanja Ćelić
AuthorNathalie Mougenot
AuthorPierre Maitrias
AuthorZiad A. Massy
AuthorAnne-Marie Lompreh
AuthorLaurent Metzinger
AuthorValérie Metzinger-Le Meuth
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Surgery
Abstract (english)
Objective: Restenosis is a frequent complication of angioplasty. It consists of a neointimal hyperplasia resulting from progression and migration of vascular smooth muscle cells (VSMC) into the vessel lumen. microRNA miR-223 has recently been shown to be involved in cardiovascular diseases including atherosclerosis, vascular calcification and arterial thrombosis. In this study, our aim was to assess the impact of miR-223 modulation on restenosis in a rat model of carotid artery after balloon injury. Methods: The over and down-expression of miR-223 was induced by adenoviral vectors, containing either a pre-miR-223 sequence allowing artificial miR-223 expression or a sponge sequence, trapping the native microRNA, respectively. Restenosis was quantified on stained rat carotid sections. Results: In vitro, three mRNA (Myocyte Enhancer Factor 2C (MEF2C), Ras homolog gene family, member B (RhoB) and Nuclear factor 1 A-type (NFIA)) reported as miR-223 direct targets and known to be implicated in VSMC differentiation and contractility were studied by RT-qPCR. Our findings showed that down-expression of miR-223 significantly reduced neointimal hyperplasia by 44% in carotids, and was associated with a 2-3- fold overexpression of MEF2C, RhoB and NFIA in a murine monocyte macrophage cell line, RAW 264.7 cells. Conclusion: Down-regulating miR-223 could be a potential therapeutic approach to prevent restenosis after angioplasty.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCurrent Vascular Pharmacology
Numberingvol. 17, no. 5, pp. 507-516
p-ISSN1570-1611
DOIhttps://doi.org/10.2174/1570161117666190705141152
URN:NBNurn:nbn:hr:184:088359
Publication2019
Document URLhttp://bib.irb.hr/1053178
Type of resourceText
Access conditionsInstitutional access
Terms of useLicence In copyright icon
Created on2022-03-15 08:25:32