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Scientific paper - Review paper
The European antibody network's practical guide to finding and validating suitable antibodies for research
mAbs, 8 (2015), 1; 27-36. https://doi.org/10.1080/19420862.2015.1100787
Roncador, Giovanna; Engel, Pablo; Maestre, Lorena; Anderson, Amanda P.; Cordell, Jacqueline L.; Cragg, Mark S.; Šerbec, Vladka Č.; Jones, Margaret; Lisnić, Vanda J.; Kremer, Leonor; Li, Demin; Koch-Nolte, Friedrich; Pascual, Núria; Rodríguez-Barbosa, Jose-Ignacio; Torensma, Ruurd; Turley, Helen; Pulford, Karen; Banham, Alison H. More authors...

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Roncador, G., Engel, P., Maestre, L., Anderson, A. P., Cordell, J. L., Cragg, M. S. ... Banham, A. H. (2015). The European antibody network's practical guide to finding and validating suitable antibodies for research. mAbs, 8. (1), 27-36. doi: 10.1080/19420862.2015.1100787

Roncador, Giovanna, et al. "The European antibody network's practical guide to finding and validating suitable antibodies for research." mAbs, vol. 8, no. 1, 2015, pp. 27-36. https://doi.org/10.1080/19420862.2015.1100787

Roncador, Giovanna, Pablo Engel, Lorena Maestre, Amanda P. Anderson, Jacqueline L. Cordell, Mark S. Cragg, Vladka Č. Šerbec, et al. "The European antibody network's practical guide to finding and validating suitable antibodies for research." mAbs 8, no. 1 (2015): 27-36. https://doi.org/10.1080/19420862.2015.1100787

Roncador, G., et al. (2015) 'The European antibody network's practical guide to finding and validating suitable antibodies for research', mAbs, 8(1), pp. 27-36. doi: 10.1080/19420862.2015.1100787

Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS, and sur.. The European antibody network's practical guide to finding and validating suitable antibodies for research. mAbs [Internet]. 2015 September 29 [cited 2025 January 03];8(1):27-36. doi: 10.1080/19420862.2015.1100787

G. Roncador, et al., "The European antibody network's practical guide to finding and validating suitable antibodies for research", mAbs, vol. 8, no. 1, pp. 27-36, September 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:110150. [Accessed: 03 January 2025]

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Metadata
Title (english)The European antibody network's practical guide to finding and validating suitable antibodies for research
AuthorGiovanna Roncador
AuthorPablo Engel
AuthorLorena Maestre
AuthorAmanda P. Anderson
AuthorJacqueline L. Cordell
AuthorMark S. Cragg
AuthorVladka Č. Šerbec
AuthorMargaret Jones
AuthorVanda J. Lisnić
AuthorLeonor Kremer
AuthorDemin Li
AuthorFriedrich Koch-Nolte
AuthorNúria Pascual
AuthorJose-Ignacio Rodríguez-Barbosa
AuthorRuurd Torensma
AuthorHelen Turley
AuthorKaren Pulford
AuthorAlison H. Banham
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.
Keywords (english)
Languageenglish
Publication typeScientific paper - Review paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titlemAbs
Numberingvol. 8, no. 1, pp. 27-36
p-ISSN1942-0862
e-ISSN1942-0870
DOIhttps://doi.org/10.1080/19420862.2015.1100787
URN:NBNurn:nbn:hr:184:110150
Publication2015-09-29
Document URLhttps://www.tandfonline.com/doi/full/10.1080/19420862.2015.1100787
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 3.0 Unported (CC BY 3.0) icon
Created on2022-03-10 17:45:52