prikaz prve stranice dokumenta Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity
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Scientific paper - Original scientific paper
Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity
DNA Repair, 90 (2020); 102848. https://doi.org/10.1016/j.dnarep.2020.102848
Svetec Miklenić, Marina; Gatalica, Nikolina; Matanović, Angela; Žunar, Bojan; Štafa, Anamarija; Lisnić, Berislav; Svetec, Ivan Krešimir

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Svetec Miklenić, M., Gatalica, N., Matanović, A., Žunar, B., Štafa, A., Lisnić, B. & Svetec, I. K. (2020). Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity. DNA Repair, 90.. doi: 10.1016/j.dnarep.2020.102848

Svetec Miklenić, Marina, et al. "Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity." DNA Repair, vol. 90, 2020. https://doi.org/10.1016/j.dnarep.2020.102848

Svetec Miklenić, Marina, Nikolina Gatalica, Angela Matanović, Bojan Žunar, Anamarija Štafa, Berislav Lisnić and Ivan Krešimir Svetec. "Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity." DNA Repair 90 (2020). https://doi.org/10.1016/j.dnarep.2020.102848

Svetec Miklenić, M., et al. (2020) 'Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity', DNA Repair, 90. doi: 10.1016/j.dnarep.2020.102848

Svetec Miklenić M, Gatalica N, Matanović A, Žunar B, Štafa A, Lisnić B, and sur.. Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity. DNA Repair [Internet]. 2020 [cited 2024 December 02];90. doi: 10.1016/j.dnarep.2020.102848

M. Svetec Miklenić, et al., "Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity", DNA Repair, vol. 90, 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:046119. [Accessed: 02 December 2024]

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Metadata
Title (english)Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity
AuthorMarina Svetec Miklenić
MBZ: 314102
AuthorNikolina Gatalica
AuthorAngela Matanović
MBZ: 383924
AuthorBojan Žunar
MBZ: 333226
AuthorAnamarija Štafa
MBZ: 262126
AuthorBerislav Lisnić
AuthorIvan Krešimir Svetec
MBZ: 210466
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Palindromic sequences in DNA can instigate genetic recombination and genome instability, which can result in devastating conditions such as the Emmanuel syndrome. Palindrome recombinogenicity increases with its size and sequence similarity between palindrome arms, while quasipalindromes with long spacers are less recombinogenic. However, the minimal spacer length, which could reduce or abolish palindrome recombinogenicity in the eukaryotic genome, was never determined. Therefore, we constructed a series of palindromes containing spacers of lengths ranging from 0 (perfect palindrome) to 10 bp and tested their recombinogenicity in yeast Saccharomyces cerevisiae. We found that a 7 bp spacer significantly reduces 126 bp palindrome recombinogenicity, while a 10 bp spacer completely stabilizes palindromes up to 150 bp long. Additionally, we showed that palindrome stimulated recombination rate is not dependent on Mus81 and Yen1 endonucleases. We also compared the recombinogenicity of a perfect 126 bp palindrome and a corresponding quasipalindrome consisting of the same palindrome arms with a stabilising 10 bp spacer in sgs1Δ and rad27Δ backgrounds, since both Sgs1 helicase and Rad27 endonuclease are implicated in preventing hairpin formation at palindromic sequences during replication.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleDNA Repair
Numberingvol. 90, 102848
p-ISSN1568-7864
DOIhttps://doi.org/10.1016/j.dnarep.2020.102848
URN:NBNurn:nbn:hr:184:046119
Publication2020
Document URLhttp://bib.irb.hr/1112340
Type of resourceText
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2022-03-02 12:29:09