prikaz prve stranice dokumenta Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes
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Scientific paper - Original scientific paper
Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes
Acta Histochemica, 120 (2018), 8; 748-756. https://doi.org/10.1016/j.acthis.2018.08.015
Vukojević, Katarina; Raguž, Fila; Saraga, Marijan; Filipović, Natalija; Bočina, Ivana; Kero, Darko; Glavina Durdov, Merica; Martinović, Vlatka; Saraga-Babić, Mirna

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Vukojević, K., Raguž, F., Saraga, M., Filipović, N., Bočina, I., Kero, D. ... Saraga Babić, M. (2018). Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes. Acta Histochemica, 120. (8), 748-756. doi: 10.1016/j.acthis.2018.08.015

Vukojević, Katarina, et al. "Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes." Acta Histochemica, vol. 120, no. 8, 2018, pp. 748-756. https://doi.org/10.1016/j.acthis.2018.08.015

Vukojević, Katarina, Fila Raguž, Marijan Saraga, Natalija Filipović, Ivana Bočina, Darko Kero, Merica Glavina Durdov, Vlatka Martinović and Mirna Saraga Babić. "Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes." Acta Histochemica 120, no. 8 (2018): 748-756. https://doi.org/10.1016/j.acthis.2018.08.015

Vukojević, K., et al. (2018) 'Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes', Acta Histochemica, 120(8), pp. 748-756. doi: 10.1016/j.acthis.2018.08.015

Vukojević K, Raguž F, Saraga M, Filipović N, Bočina I, Kero D, and sur.. Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes. Acta Histochemica [Internet]. 2018 [cited 2025 April 11];120(8):748-756. doi: 10.1016/j.acthis.2018.08.015

K. Vukojević, et al., "Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes", Acta Histochemica, vol. 120, no. 8, pp. 748-756, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:446598. [Accessed: 11 April 2025]

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Metadata
Title (english)Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes
AuthorKatarina Vukojević
MBZ: 287964
AuthorFila Raguž
AuthorMarijan Saraga
MBZ: 182991
AuthorNatalija Filipović
MBZ: 264494
AuthorIvana Bočina
MBZ: 210014
AuthorDarko Kero
MBZ: 346653
AuthorMerica Glavina Durdov
MBZ: 207682
AuthorVlatka Martinović
MBZ: 249565
AuthorMirna Saraga-Babić
MBZ: 111141
Author's institutionUniversity of Rijeka
Faculty of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Background: Podocytes are postmitotic, highly specialized cells which maintain the glomerular filtration barrier (GFB). Their injury is characterized by foot processes effacement and change in protein expression leading to proteinuria and end-stage kidney disease. Methods: Our study focuses on the morphological and immunohistochemical changes of human podocytes during normal development and postnatal period, compared to congenital nephrotic syndrome of the Finnish type (CNF). Kidney tissues... More taken from 17 human conceptuses 8th-38th weeks old, two healthy and three CNF kidneys were embedded in paraffin for immunohistochemical or double immunofluorescence methods, or were embedded in resin for electron microscopy. Paraffin sections were stained with markers for proliferation (Ki-67), proteins nephrin and nestin, and alpha-tubulin. Quantification of positive cells were performed using Mann Whitney and Kruskal-Wallis test. Results: Tissue analysis showed that proliferation of podocytes gradually decreased during development and disappeared in postnatal period. Decrease in number of ciliated glomerular cells and visceral podocytes (from 47% to 3%), and parietal epithelial cells (from 32% to 7%) characterized normal development. Nestin and nephrin co-expressed in developing podocytes in different cellular compartments. During development, nephrin expression increased (from 17% to 75%) and postnatally changed its pattern, while nestin positive glomerular cells decreased from 98% to 40%. CNF glomeruli displayed increased number of immature ciliated podocytes (6%) and parietal epithelial cells (9%). Conclusion: Changes in cytoplasmic alpha-tubulin expression and reduced nephrin expression (20%) indicating association of incomplete podocyte maturation with failure of GFB function and appearance of prenatal proteinuria in CNF patients. Less
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionAccepted version
Journal titleActa Histochemica
Numberingvol. 120, no. 8, pp. 748-756
p-ISSN0065-1281
DOIhttps://doi.org/10.1016/j.acthis.2018.08.015
URN:NBNurn:nbn:hr:184:446598
Publication2018
ProjectNumber: 216-2160528-0507
Title (croatian): Genski izražaj u ranom razvoju čovjeka
Title (english): Gene expression in early human development
Leader: Mirna Saraga-Babić
Jurisdiction: Croatia
Funding stream: ZP
ProjectNumber: IP-2016-06-2575
Title (croatian): Karakterizacija kandidat gena za kongenitalne anomalije bubrega i urotrakta (CAKUT) tijekom razvoja u miša i čovjeka
Acronym: CAKUT
Leader: Katarina Vukojević
Jurisdiction: Croatia
Funding stream: IP
Document URLhttp://bib.irb.hr/951901
Type of resourceText
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2022-02-22 16:20:42
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