prikaz prve stranice dokumenta Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol
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Scientific paper - Original scientific paper
Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol
Infection and Immunity, 76 (2008), 6; 2671-2677. https://doi.org/10.1128/IAI.00185-08
Šantić, Marina; Asare, Rexford; Škrobonja, Ivana; Jones, Snake; Abu Kwaik, Yousef

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Šantić, M., Asare, R., Škrobonja, I., Jones, S. & Abu Kwaik, Y. (2008). Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol. Infection and Immunity, 76. (6), 2671-2677. doi: 10.1128/IAI.00185-08

Šantić, Marina, et al. "Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol." Infection and Immunity, vol. 76, no. 6, 2008, pp. 2671-2677. https://doi.org/10.1128/IAI.00185-08

Šantić, Marina, Rexford Asare, Ivana Škrobonja, Snake Jones and Yousef Abu Kwaik. "Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol." Infection and Immunity 76, no. 6 (2008): 2671-2677. https://doi.org/10.1128/IAI.00185-08

Šantić, M., et al. (2008) 'Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol', Infection and Immunity, 76(6), pp. 2671-2677. doi: 10.1128/IAI.00185-08

Šantić M, Asare R, Škrobonja I, Jones S, Abu Kwaik Y. Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol. Infection and Immunity [Internet]. 2008 June [cited 2025 April 08];76(6):2671-2677. doi: 10.1128/IAI.00185-08

M. Šantić, R. Asare, I. Škrobonja, S. Jones and Y. Abu Kwaik, "Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol", Infection and Immunity, vol. 76, no. 6, pp. 2671-2677, June 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:789646. [Accessed: 08 April 2025]

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Metadata
Title (english)Acquisition of the vacuolar ATPase proton pump and phagosome acidification are essential for escape of Francisella tularensis into the macrophage cytosol
AuthorMarina Šantić
AuthorRexford Asare
AuthorIvana Škrobonja
AuthorSnake Jones
AuthorYousef Abu Kwaik
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
ABSTRACT The -containing phagosome (FCP) matures to a late-endosome-like phagosome prior to bacterial escape into the cytosols of macrophages, where bacterial proliferation occurs. Our data show that within the first 15 min after infection of primary human monocyte-derived macrophages (hMDMs), ∼90% of the FCPs acquire the proton vacuolar ATPase (vATPase) pump and the lysomotropic dye LysoTracker, which concentrates in acidic... More compartments, similar to phagosomes harboring the control. The acquired proton vATPase pump and lysomotropic dye are gradually lost by 30 to 60 min postinfection, which coincides with bacterial escape into the cytosols of hMDMs. Colocalization of phagosomes harboring the mutant with the vATPase pump and the LysoTracker dye was also transient, and the loss of colocalization was faster than that observed for the wild-type strain, which is consistent with the faster escape of the mutant into the macrophage cytosol. In contrast, colocalization of both makers with phagosomes harboring the mutant was persistent, which is consistent with fusion to the lysosomes and failure of the mutant to escape into the macrophage cytosol. We have utilized a fluorescence microscopy-based phagosome integrity assay for differential labeling of vacuolar versus cytosolic bacteria, using antibacterial antibodies loaded into the cytosols of live hMDMs. We show that specific inhibition of the proton vATPase pump by bafilomycin A1 (BFA) blocks rapid bacterial escape into the cytosols of hMDMs, but 30% to 50% of the bacteria escape into the cytosol by 6 to 12 h after BFA treatment. The effect of BFA on the blocking of bacterial escape into the cytosol is completely reversible, as the bacteria escape after removal of BFA. We also show that the limited fusion of the FCP to lysosomes is not due to failure to recruit the late-endosomal fusion regulator Rab7. Therefore, within few minutes of its biogenesis, the FCP transiently acquires the proton vATPase pump to acidify the phagosome, and this transient acidification is essential for subsequent bacterial escape into the macrophage cytosol. Less
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInfection and Immunity
Numberingvol. 76, no. 6, pp. 2671-2677
p-ISSN0019-9567
e-ISSN1098-5522
DOIhttps://doi.org/10.1128/IAI.00185-08
URN:NBNurn:nbn:hr:184:789646
Publication2008-06
Document URLhttps://journals.asm.org/doi/10.1128/IAI.00185-08
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-01-28 10:44:53
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