prikaz prve stranice dokumenta Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
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Scientific paper - Original scientific paper
Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
Frontiers in Cellular and Infection Microbiology, 8 (2018); 111. https://doi.org/10.3389/fcimb.2018.00111
Spidlova, Petra; Stojkova, Pavla; Dankova, Vera; Senitkova, Iva; Šantić, Marina; Pinkas, Dominik; Philimonenko, Vlada; Stulik, Jiri

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Spidlova, P., Stojkova, P., Dankova, V., Senitkova, I., Šantić, M., Pinkas, D. ... Stulik, J. (2018). Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity. Frontiers in Cellular and Infection Microbiology, 8.. doi: 10.3389/fcimb.2018.00111

Spidlova, Petra, et al. "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity." Frontiers in Cellular and Infection Microbiology, vol. 8, 2018. https://doi.org/10.3389/fcimb.2018.00111

Spidlova, Petra, Pavla Stojkova, Vera Dankova, Iva Senitkova, Marina Šantić, Dominik Pinkas, Vlada Philimonenko and Jiri Stulik. "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity." Frontiers in Cellular and Infection Microbiology 8 (2018). https://doi.org/10.3389/fcimb.2018.00111

Spidlova, P., et al. (2018) 'Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity', Frontiers in Cellular and Infection Microbiology, 8. doi: 10.3389/fcimb.2018.00111

Spidlova P, Stojkova P, Dankova V, Senitkova I, Šantić M, Pinkas D, and sur.. Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity. Frontiers in Cellular and Infection Microbiology [Internet]. 2018 April 10 [cited 2024 December 24];8. doi: 10.3389/fcimb.2018.00111

P. Spidlova, et al., "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity", Frontiers in Cellular and Infection Microbiology, vol. 8, April 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:851493. [Accessed: 24 December 2024]

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Title (english)Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
AuthorPetra Spidlova
AuthorPavla Stojkova
AuthorVera Dankova
AuthorIva Senitkova
AuthorMarina Šantić
AuthorDominik Pinkas
AuthorVlada Philimonenko
AuthorJiri Stulik
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
D-alanyl-D-alanine carboxypeptidase, product of dacD gene in Francisella, belongs to penicillin binding proteins (PBPs) and is involved in remodeling of newly synthetized peptidoglycan. In E. coli, PBPs are synthetized in various growth phases and they are able to substitute each other to a certain extent. The DacD protein was found to be accumulated in fraction enriched in membrane proteins from severely attenuated dsbA deletion mutant strain. It has been presumed that the DsbA is not a virulence factor by itself but that its substrates, whose correct folding and topology are dependent on the DsbA oxidoreductase and/or isomerase activities, are the primary virulence factors. Here we demonstrate that Francisella DacD is required for intracellular replication and virulence in mice. The dacD insertion mutant strain showed higher sensitivity to acidic pH, high temperature and high osmolarity when compared to the wild-type. Eventually, transmission electron microscopy revealed differences in mutant bacteria in both the size and defects in outer membrane underlying its SDS and serum sensitivity. Taken together these results suggest DacD plays an important role in Francisella pathogenicity.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleFrontiers in Cellular and Infection Microbiology
Numberingvol. 8, 111
e-ISSN2235-2988
DOIhttps://doi.org/10.3389/fcimb.2018.00111
URN:NBNurn:nbn:hr:184:851493
Publication2018-04-10
ProjectNumber: IP-2016-06-9003
Title (croatian): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Acronym: FRANCYCLE
Leader: Marina Šantić
Jurisdiction: Croatia
Funding stream: IP
Document URLhttp://journal.frontiersin.org/article/10.3389/fcimb.2018.00111/full
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-01-28 09:11:05