prikaz prve stranice dokumenta Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
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Scientific paper - Review paper
Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
Biomedicines, 9 (2021), 6; 603. https://doi.org/10.3390/biomedicines9060603
Malenica, Mladenka; Vukomanović, Marija; Kurtjak, Mario; Masciotti, Valentina; dal Zilio, Simone; Greco, Silvio; Lazzarino, Marco; Krušić, Vedrana; Perčić, Marko; Jelovica Badovinac, Ivana; Wechtersbach, Karmen; Vidović, Ivona; Baričević, Vanja; Valić, Srećko; Lučin, Pero; Kojc, Nika; Grabušić, Kristina More authors...

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Malenica, M., Vukomanović, M., Kurtjak, M., Masciotti, V., dal Zilio, S., Greco, S. ... Grabušić, K. (2021). Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids. Biomedicines, 9. (6). doi: 10.3390/biomedicines9060603

Malenica, Mladenka, et al. "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids." Biomedicines, vol. 9, no. 6, 2021. https://doi.org/10.3390/biomedicines9060603

Malenica, Mladenka, Marija Vukomanović, Mario Kurtjak, Valentina Masciotti, Simone dal Zilio, Silvio Greco, Marco Lazzarino, et al. "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids." Biomedicines 9, no. 6 (2021). https://doi.org/10.3390/biomedicines9060603

Malenica, M., et al. (2021) 'Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids', Biomedicines, 9(6). doi: 10.3390/biomedicines9060603

Malenica M, Vukomanović M, Kurtjak M, Masciotti V, dal Zilio S, Greco S, and sur.. Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids. Biomedicines [Internet]. 2021 May 26 [cited 2024 December 13];9(6). doi: 10.3390/biomedicines9060603

M. Malenica, et al., "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids", Biomedicines, vol. 9, no. 6, May 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:323752. [Accessed: 13 December 2024]

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Title (english)Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
AuthorMladenka Malenica
AuthorMarija Vukomanović
AuthorMario Kurtjak
AuthorValentina Masciotti
AuthorSimone dal Zilio
AuthorSilvio Greco
AuthorMarco Lazzarino
AuthorVedrana Krušić
AuthorMarko Perčić
AuthorIvana Jelovica Badovinac
AuthorKarmen Wechtersbach
AuthorIvona Vidović
AuthorVanja Baričević
AuthorSrećko Valić
AuthorPero Lučin
AuthorNika Kojc
AuthorKristina Grabušić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. In addition, we discuss approaches for EV imaging and identify the potential benefits and disadvantages when different AFM and EM methods are applied, including numerous factors that influence the morphological characterisation, standardisation, or formation of artefacts. We also demonstrate the effects of some of these factors by using cerebrospinal fluid as an example of human biofluid with a simpler composition. Here presented comparison of approaches to EV imaging should help to estimate the current state in morphology research of EVs from human biofluids and to identify the most efficient pathways towards the standardisation of sample preparation and microscopy modes.
Keywords (english)
Languageenglish
Publication typeScientific paper - Review paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBiomedicines
Numberingvol. 9, no. 6, 603
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines9060603
URN:NBNurn:nbn:hr:184:323752
Publication2021-05-26
Document URLhttps://www.mdpi.com/2227-9059/9/6/603
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-12-03 11:33:26