| Abstract | Povećana rezistentnost patogenih bakterijskih sojeva na široko primjenjivane antibiotike predstavlja globalni problem s kojim se sureće moderna medicina prvenstveno u suzbijanju i liječenju hospitalnih infekcija. Potraga za alternativnim rješenjima svrstala je odreĎene vrste meda sa izrazitim antibakterijskim djelovanjem u skupinu tvari s potencijalnim antibiotskim učincima. U ovom istraţivanju ispitivano je antibakterijsko djelovanje ukupno 52 uzorka meda svrstanih u 5 monoflornih grupa prema potvrĎenom botaničkom podrijetlu: lipa (Tilia sp)., kadulja (Salvia officinalis L.), bagrem (Robinia pseudoacacia L), kesten (Castanea sativa Mill.) i medun te po jednog uzorka meda manuke, tzv. „umjetnog― meda te antibiotika, uz pomoć makro- i mikro-diluciijske metode. Uzorci su podrijetlom s različitih lokacija pčelinjaka širom Republike Hrvatske (Hrvatsko primorje s otocima Krk, Cres, Lošinj i Pag, Istra, Gorski kotar, Ogulinsko-plaščanska udolina, Lika, područje zagrebačkog prstena, Pokuplje, Banovina, MeĎimurje, Zapadna Slavonija, Baranja). Ispitivanje bakteriostatskog (MIK) i baktericidnog (MBK) učinka provedeno je na 6 sojeva gram pozitivnih bakterija (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae, ESBL Escherichia coli) i 3 soja gram negativnih bakterija (Staphylococcus aureus, meticilin-rezistentan Staphylococcus aureus (MRSA), Enterococcus faecium). Visoku razinu antibakterijskog djelovanja djelovanja na sve ispitivane bakterije ostvario je uzorak meduna (M8) od kojih se posebice ističe bakteriostatski učinak na rezistentan soj MRSA u koncentraciji od 0,05 mg/kg (3,1% v/v), dok za isti učinak potrebna koncentracija antibiotika je 4 puta veća i iznosi 0,2 mg/kg (12,5% v/v). Pojedini medovi od bagrema, lipe, kadulje, te uzorci tzv. „umjetnog― i meda manuke pripadaju skupini sa niskom razinom antibakterijskog djelovanja. |
| Abstract (english) | Increased resistance of pathogenic bacterial strains to widely used antibiotics represents a global problem faced by modern medicine primarily in the control and treatment of nosocomial infections. The search for alternative solutions has classified particular types of honey with a pronounced antibacterial effect into a group of substances with potential antibiotic effects. A total of 52 honey samples were studied for their antibacterial activity classified in 5 unifloral groups by their confirmed botanical origin: linden (Tilia sp.), sage (S. officinalis L.), black locust (R. pseudoacacia L), chestnut (C. sativa Mill.) and honeydew honey, one sample per each of manuka and the so-called "artificial" honey as well as the antibiotics by means of macro- and micro-dilution methods). Samples derived from different apiary locations of Croatia (Croatian Littoral with the islands of Krk, Cres, Lošinj and Pag), Istria, Gorski kotar, Ogulin-Plaški valley, Lika, wider District of Zagreb, Pokuplje, Banovina, MeĎimurje, Western Slavonia and Baranja). The study of the bacteriostatic (MIK) and bactericidal (MBK) effect of studied honey samples was carried out on 6 strains of gram-positive bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae, ESBL Escherichia cphylococci and Escherichia coli negative), methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecium). High level of antibacterial activity on all tested bacteria was reached by the honeydew honey sample (M8), with particular bacteriostatic effect on the tested MRSA strain in the concentration of 0.05 mg/kg (3.1% v/v), whilst the required concentration of antibiotics for the same effect was 4 times higher amounting 0.2 mg/kg (12.5% v/v). Particular acacia, linden and sage honey samples, as well as the samples of "artificial" and manuka honey, fall into the group of samples showing a low level of antibacterial activity. |
