Escherichia coli is gram negative, oxidase negative, catalase positive optional anaerobic bacillus. It is known that some strains of E. coli form a dominant part of the human intestinal microflora and contribute to the normal physiology of the digestive system. On the other hand, some strains of E. coli cause infections of the digestive and urinary systems. The most common strains of. E. coli that cause gastrointestinal infections are Enterotoxic E. coli ,Enteropathogenic E. coli , Enteroinvasive E. coli, and Enterohemorrhagic E. coli , while Uropathogenic E. coli is the most common cause of urinary tract infections. E. coli is considered a mesophilic bacterium and temperatures of 20-40 °C are favorable for its growth and reproduction. Previous research has shown that E. coli reproduces successfully at temperatures below 30 °C and that it can survive longer in nature at lower than at higher temperatures in relation to the optimal growth temperature. Also, previous research has shown that some strains of E. coli are more suitable for reproduction fluctuating than constant temperatures. The purpose of this final work is to examine the influence of temperature on the growth kinetics of E. coli in samples of milk and l yogurt. The growth kinetics of E. coli were examined at three different temperatures (4 °C, 25 °C, 37 °C) during a period of ten days. The number of bacteria in milk and yogurt samples was monitored at 0, 3, 5, 7, and 10 days after inoculation. TBX agar is used to determine the number of bacteria. Bacterial suspensions were diluted tenfold immediately prior to inoculation on TBX agar, and then the agars were incubated at 37 °C for 24 hours, after which time the number of bacteria was read. The results of this study show that E. coli grows in milk at 4 °C, 25 °C and 37 °C, while E. coli grows in yogurt at a temperature of 25 °C and 37 °C, and E. coli does not have the ability to reproduce at a temperature of 4 °C. It has also been shown that E. coli reproduces better in milk and that bacteria grows the best at 25 °C.