prikaz prve stranice dokumenta Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma
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Scientific paper - Original scientific paper
Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma
Croatica Chemica Acta, 89 (2016), 2; 203-211. https://doi.org/10.5562/cca2815
Trbojević-Akmačić, Irena; Nemec, Blaž; Vidic, Urška; Malić, Suzana; Miklić, Karmela; Černigoj, Urh; Vidič, Jana; Lendero Krajnc, Nika; Štrancar, Aleš; Lauc, Gordan; Lenac Roviš, Tihana; Pučić-Baković, Maja

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Trbojević Akmačić, I., Nemec, B., Vidic, U., Malić, S., Miklić, K., Černigoj, U. ... Pučić Baković, M. (2016). Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma. Croatica Chemica Acta, 89. (2), 203-211. doi: 10.5562/cca2815

Trbojević Akmačić, Irena, et al. "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma." Croatica Chemica Acta, vol. 89, no. 2, 2016, pp. 203-211. https://doi.org/10.5562/cca2815

Trbojević Akmačić, Irena, Blaž Nemec, Urška Vidic, Suzana Malić, Karmela Miklić, Urh Černigoj, Jana Vidič, et al. "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma." Croatica Chemica Acta 89, no. 2 (2016): 203-211. https://doi.org/10.5562/cca2815

Trbojević Akmačić, I., et al. (2016) 'Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma', Croatica Chemica Acta, 89(2), pp. 203-211. doi: 10.5562/cca2815

Trbojević Akmačić I, Nemec B, Vidic U, Malić S, Miklić K, Černigoj U, and sur.. Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma. Croatica Chemica Acta [Internet]. 2016 [cited 2024 November 14];89(2):203-211. doi: 10.5562/cca2815

I. Trbojević Akmačić, et al., "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma", Croatica Chemica Acta, vol. 89, no. 2, pp. 203-211, 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:695580. [Accessed: 14 November 2024]

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Metadata
Title (english)Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma
AuthorIrena Trbojević-Akmačić
MBZ: 351184
AuthorBlaž Nemec
AuthorUrška Vidic
AuthorSuzana Malić
AuthorKarmela Miklić
AuthorUrh Černigoj
AuthorJana Vidič
AuthorNika Lendero Krajnc
AuthorAleš Štrancar
AuthorGordan Lauc
MBZ: 201852
AuthorTihana Lenac Roviš
AuthorMaja Pučić-Baković
MBZ: 310572
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Changes in protein glycosylation are related to different diseases and have a potential as diagnostic and prognostic disease biomarkers. Transferrin (Tf) glycosylation changes are common marker for congenital disorders of glycosylation. However, biological interindividual variability of Tf N-glycosylation and genes involved in glycosylation regulation are not known. Therefore, high-throughput Tf isolation method and large scale glycosylation studies are needed in order to address these questions. Due to their unique chromatographic properties, the use of chromatographic monoliths enables very fast analysis cycle, thus significantly increasing sample preparation throughput. Here, we are describing characterization of novel immunoaffinity-based monolithic columns in a 96-well plate format for specific high-throughput purification of human Tf from blood plasma. We optimized the isolation and glycan preparation procedure for subsequent ultra performance liquid chromatography (UPLC) analysis of Tf N-glycosylation and managed to increase the sensitivity for approximately three times compared to initial experimental conditions, with very good reproducibility.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatica Chemica Acta
Numberingvol. 89, no. 2, pp. 203-211
p-ISSN0011-1643
DOIhttps://doi.org/10.5562/cca2815
URN:NBNurn:nbn:hr:184:695580
Publication2016
ProjectNumber: 278535
Title (croatian): Methods for high-throughput (HTP) analysis of protein glycosylation
Acronym: HIGHGLYCAN
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 324400
Title (croatian): Methods for high-throughput glycoproteomic analysis
Acronym: HTP-GLYCOMET
Jurisdiction: eu
Funding stream: FP7
Document URLhttp://bib.irb.hr/1002539
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-04-19 14:18:45