prikaz prve stranice dokumenta Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
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Scientific paper - Original scientific paper
Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
Croatian Medical Journal, 60 (2019), 3; 201-211. https://doi.org/10.3325/cmj.2019.60.201
Rod, Eduard; Matić, Igor; Antunović, Maja; Vetma, Vesna; Pavičić, Ivan; Hudetz, Damir; Marijanović, Inga; Primorac, Dragan; Ivković, Alan

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Rod, E., Matić, I., Antunović, M., Vetma, V., Pavičić, I., Hudetz, D. ... Ivković, A. (2019). Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing. Croatian Medical Journal, 60. (3), 201-211. doi: 10.3325/cmj.2019.60.201

Rod, Eduard, et al. "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing." Croatian Medical Journal, vol. 60, no. 3, 2019, pp. 201-211. https://doi.org/10.3325/cmj.2019.60.201

Rod, Eduard, Igor Matić, Maja Antunović, Vesna Vetma, Ivan Pavičić, Damir Hudetz, Inga Marijanović, Dragan Primorac and Alan Ivković. "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing." Croatian Medical Journal 60, no. 3 (2019): 201-211. https://doi.org/10.3325/cmj.2019.60.201

Rod, E., et al. (2019) 'Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing', Croatian Medical Journal, 60(3), pp. 201-211. doi: 10.3325/cmj.2019.60.201

Rod E, Matić I, Antunović M, Vetma V, Pavičić I, Hudetz D, and sur.. Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing. Croatian Medical Journal [Internet]. 2019 [cited 2025 January 02];60(3):201-211. doi: 10.3325/cmj.2019.60.201

E. Rod, et al., "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing", Croatian Medical Journal, vol. 60, no. 3, pp. 201-211, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:722027. [Accessed: 02 January 2025]

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Metadata
Title (english)Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
AuthorEduard Rod
AuthorIgor Matić
AuthorMaja Antunović
AuthorVesna Vetma
AuthorIvan Pavičić
AuthorDamir Hudetz
AuthorInga Marijanović
AuthorDragan Primorac
AuthorAlan Ivković
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Biology and Medical Genetics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract (english)
Aim To assess whether an adenoviral vector carrying the bone morphogenetic protein genes (Ad.BMP-2) can transduce human muscle tissue and direct it toward osteogenic differentiation within one hour. Methods This in vitro study, performed at the Department of Molecular Biology, Faculty of Science, Zagreb from 2012 to 2017, used human muscle tissue samples collected during anterior cruciate ligament reconstructions performed in St Catherine Hospital, Zabok. Samples from 28 patients were transduced with adenoviral vector carrying firefly luciferase cDNA (Ad.luc) by using different doses and times of transduction, and with addition of positive ions for transduction enhancement. The optimized protocol was further tested on muscle samples from three new patients, which were transduced with Ad.BMP-2. Released bone morphogenetic protein 2 (BMP-2) levels in osteogenic medium were measured every three days during a period of 21 days. Expression of osteogenic markers was measured at day 14 and 21. After 21 days of cultivation, muscle tissue was immunohistochemically stained for collagen type I detection (COL-I). Results The new transduction protocol was established using 108 plaque-forming units (P < 0.001) as an optimal dose of adenoviral vector and 30 minutes (P < 0.001) as an optimal contact time. Positive ions did not enhance transduction. Samples transduced with Ad.BMP-2 according to the optimized protocol showed enhanced expression of osteogenic markers (P < 0.050), BMP-2 (P < 0.001), and COL I. Conclusion This study confirms that Ad.BMP-2 can transduce human muscle tissue and direct it toward osteogenic differentiation within 30 minutes.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatian Medical Journal
Numberingvol. 60, no. 3, pp. 201-211
p-ISSN0353-9504
e-ISSN1332-8166
DOIhttps://doi.org/10.3325/cmj.2019.60.201
URN:NBNurn:nbn:hr:184:722027
Publication2019
Document URLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563169/
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2020-03-30 06:34:27