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master's thesis
ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI
2019. urn:nbn:hr:184:822958
Lacković, Alojzije
University of Rijeka
Faculty of Medicine
Department of Anatomy

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Lacković, A. (2019). ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:822958

Lacković, Alojzije. "ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI." Master's thesis, University of Rijeka, Faculty of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:184:822958

Lacković, Alojzije. "ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI." Master's thesis, University of Rijeka, Faculty of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:184:822958

Lacković, A. (2019). 'ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 11 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:822958

Lacković A. ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2019 [cited 2024 November 11] Available at: https://urn.nsk.hr/urn:nbn:hr:184:822958

A. Lacković, "ULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:184:822958

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Metadata
TitleULOGA KOŠTANOG MORFOGENETSKOG PROTEINA 7 U OČUVANJU TKIVA BUBREGA PRI TOPLOJ I HLADNOJ ISHEMIJI
Title (english)The role of bone morphogenetic protein 7 in the preservation of kidney tissue in warm and cold ischemia
AuthorAlojzije Lacković
MentorTanja Ćelić (mentor)
Committee memberJosip Španjol (predsjednik povjerenstva)
Committee memberHrvoje Jakovac (član povjerenstva)
Committee memberOlga Peloza (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Rijeka
Defense date and country2019-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Urology
Abstract
Ishemijsko-reperfuzijska ozljeda jedan je od najvažnijih uzroka oslabljene funkcije transplantiranog bubrega. Dosad su identificirani mnogi čimbenici, koji mogu povećati vjerojatnost njezina nastanka, a jedan od najvažnijih je način skladištenja bubrega, odnosno duljina trajanja hladne ishemije. Osnovni uzrok oštećenja tkiva u hladnoj ishemiji je nedostatak kisika, koji dovodi do smanjenja koncentracije adenozin trifosfata (engl. adenosine triphosphate, ATP-a) te slabljenja aktivnosti Na/K-ATP-aze (Na/K crpke). Puno kompleksniji i teži oblik oštećenja tkiva nastaje ponovnom uspostavom cirkulacije, odnosno reperfuzijom, prilikom koje dolazi do pojačanog ulaska kisika u stanice te stvaranja velike količine slobodnih kisikovih radikala. Njihovo nakupljanje unutar tkiva uzrokuje oštećenja DNA, proteina i lipida stanične membrane. Ljudski organizam djelomično je zaštićen od djelovanja slobodnih kisikovih radikala pomoću vlastitih antioksidativnih enzimatskih sustava, poput superoksid dismutaze (SOD), glutation peroksidaze (engl. gluthatione peroxidase, GSH-Px) te katalaze. Kako bi se spriječio nastanak navedenih oštećenja, u kliničkoj se praksi za vrijeme trajanja ishemije bubrega koriste različite otopine za njegovo čuvanje. Cilj ovog rada bio je ispitati da li primjena koštanog morfogenetskog proetina 7 (engl. bone morphogenetic protein 7, BMP-7) smanjuje oštećenje tkiva bubrega tijekom hladne i tople ishemije uspješnije od UW otopine te da li je dodavanjem BMP-7 u UW otopinu oštećenje tkiva manje nego kada se tkivo ispire samo s UW otopinom. Rezultati ovog istraživanja pokazali su kako primjena rhBMP-7 tijekom hladne i tople ishemije, uspješnije čuva tkivo bubrega od oksidativnog oštećenja proteina i lipida te NA+/K+ ATP-aze u odnosu na UW otopinu, dok istovremeno ne postoji značajnija razlika u aktivnosti antioksidativnih enzima, GSH-Px te SOD.
Abstract (english)
Ischemic-reperfusion injury is important cause of transplanted kidney decreased function. So far many risk factors have been identified, which may increase the probability of its occurrence, the most important one is duration of the cold ischemia. The main cause of tissue damage in cold ischemia is lack of oxygen, which leads to a decreased concentration of adenosine triphosphate (ATP) and decrease in Na/K-ATPase activity. More complex and severe form of tissue damage is caused by re-establishment of circulation, known as reperfusion, which leads to increased oxygen level in the cells and creation of reactive oxygen species (ROS). ROS cause damage of the DNA, proteins, and lipids of cell membrane. Human body is partially protected from effects of ROS by its own antioxidant enzymatic systems, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase. In order to prevent occurrence of these impairments, different organ preservation solutions can be used during cold ischemia period. Aim of this study was to examine whether the application of bone morphogenetic protein 7 (BMP-7) reduces kidney damage during cold and warm ischemia more successfully than UW solution, and whether the adding of BMP-7 into UW solution will prevent tissue damage more efficiently than UW solution itself. The results of this study demonstrated that rhBMP-7, compared with UW solution, better preserves kidney tissue after cold and warm ischemia, decreasing the levels of LPO, PCC and preserving the damaging of NA+/K+ ATP-ase. However, there was no significant difference in antioxidative enzymes activity levels between these two groups.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:822958
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-01-20 18:30:33