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undergraduate thesis
UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM
2018. urn:nbn:hr:184:813088
Fućak, Petra
University of Rijeka
Faculty of Medicine
Department of Microbiology and Parasitology

Cite this document

Fućak, P. (2018). UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:813088

Fućak, Petra. "UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:813088

Fućak, Petra. "UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:813088

Fućak, P. (2018). 'UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM', Undergraduate thesis, University of Rijeka, Faculty of Medicine, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:813088

Fućak P. UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:184:813088

P. Fućak, "UTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM", Undergraduate thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:813088

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Metadata
TitleUTJECAJ TEMPERATURE NA RAZMNOŽAVANJE FRANCISELLA NOVICIDA U DICTYOSTELIUM DISCOIDEUM
AuthorPetra Fućak
MentorMarina Šantić (mentor)
Committee memberMaja Abram (predsjednik povjerenstva)
Committee memberMarin Tota (član povjerenstva)
Committee memberMarina Šantić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Rijeka
Defense date and country2018-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Francisella tularensis je gram-negativna aerobna, asporogena bakterija. Pripada porodici Francisellaceae. Do danas su poznate tri podvrste F. tularensis koje se međusobno razlikuju u virulenciji, te zemljopisnom podrijetlu a to su: F. tularensis (tip A), F. holarctica (tip B), F. mediasiatica. Tip A i tip B uzrokuju tularemiju. Simptomi tularemije su groznica, glavobolja, osjećaj slabosti, povraćanje, mučnina. Infektivna doza je vrlo niska, potrebno je samo 10 bakterija kako bi se razvila bolest. Inficirati se možemo preko kontaminirane hrane, vode, ali i udisanjem kontaminiranog aerosola. F. tularensis se već godinama smatra potencijalnim biološkim oružjem. F. novicida smatra se rijetkim patogenom, a do razvoja bolesti dolazi kod imunokompromitiranih ljudi. Dictyostelium discoideum je jednostanični eukariot koja pripada rodu Amoebozoa, a živi u gornjem sloju tla koji je bogat organskim materijalom. Kada nastupe nepovoljni uvjeti (gladovanje, nedostatak vode) stanice se počinju nakupljati i stvara se višestanično plodno tijelo. Plodno tijelo se sastoji se od stabljike i sorusa. Unutar plodnog tijela se nalaze spore koje su zaštićene staničnim zidom. D. discoideum se može reproducirati na 3 načina: spolno, nespolno i vegetativno-mitotički. Cilj ovog rada je praćenje utjecaja različith temperatura na rast i razmnožavanje F. novicida unutar D. discoideum. Kinetika rasta i razmnožavanja F. novicida u D. discoideum je praćena u razdobljima od 2, 4, 24, 48 i 72 sata nakon infekcije. Broj unutarstaničnih i izvanstaničnih bakterija je određen nasađivanjem deseterostrukih razjeđenja na BCYE agar. Rezultati pokazuju da se F. novicida najbolje razmnožava unutar D. discoideum na temperaturi od 30 ºC.
Abstract (english)
Francisella tularensis is gram-negative anaerobic, non spore forming bacteria. There are three known F. tularensis subspecies today that differ in virulence and geographical origin, F. tularensis (type A), F. holarctica (type B), F. mediasiatica. Type A and type B cause tularemia. Symptoms of Tularemia are fever, headache, weaknes, vomiting and nausea. Minimal infective dose is 10. We can get infected from contaminated food, water and from inhaling aerosol. F. tularensis is considered to be a biological weapon for years now. It is considered to be a rare pathogen and the development of the disease occurs in immunocompromised people. Dictyostelium discoideum is a unicellular eukaryote, living below the soil surface rich in organic material, belonging to the phylum Amoebozoa. In unfavourable conditions, the cells begin to accumulate in multicellular fertile body. Fertile body is comprised of stem and sorus. Spores under body are protected by cellular wall. D. discoideum can reproduce in three ways: sexually, nonsexually and by vegetative propagation. The aim of this study was to monitor influence of different temperatures on the growth and replication of F. novicida in D. discoideum. The growth kinetics of F. novicida in D. discoideum were monitored in periods of 2, 4, 24, 48 and 72 hours after infection. The number of intracellular and extracellular bacteria was determined by tenfold dilution BCYE agar. The results show that F. novicida best replicates in D. discoideum at temperatures of 30°C.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:813088
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-12-07 08:37:17