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undergraduate thesis
Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6
2018. urn:nbn:hr:184:865056
Kajtazi, Ardiana
University of Rijeka
Faculty of Medicine
Department of Molecular Medicine and Biotechnology

Cite this document

Kajtazi, A. (2018). Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6 (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:865056

Kajtazi, Ardiana. "Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:865056

Kajtazi, Ardiana. "Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:865056

Kajtazi, A. (2018). 'Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6', Undergraduate thesis, University of Rijeka, Faculty of Medicine, accessed 23 October 2024, https://urn.nsk.hr/urn:nbn:hr:184:865056

Kajtazi A. Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6 [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 October 23] Available at: https://urn.nsk.hr/urn:nbn:hr:184:865056

A. Kajtazi, "Uporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6", Undergraduate thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:865056

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Metadata
TitleUporaba specifičnih siRNK molekula za spriječavanje izražaja ribosomskog proteina S6
Title (english)The use of specific siRNA molecules in the prevetion of expression of ribosomal protein S6
AuthorArdiana Kajtazi
MentorSlađana Bursać (mentor)
Committee memberLara Batičić Pučar (predsjednik povjerenstva)
Committee memberDijana Detel (član povjerenstva)
Committee memberSlađana Bursać (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Molecular Medicine and Biotechnology)
Rijeka
Defense date and country2018-07-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Proučavanje gena i njihove funkcije vrlo je značajno kako bi se dobio uvid u molekularne mehanizme koji dovode do nastanka bolesti u ljudi. Upotreba malih interferirajućih RNK, siRNA, ima veliku važnost u proučavanju funkcije gena i vrlo se uspješno primjenjuje u postupcima manipulacije gena. One se uspješno koriste za sprječavanje izražaja gena na razini gRNK čime ometaju njenu translaciju u protein. U ovom radu testirana je učinkovitost utišavanja ribosomskog proteina S6 (RPS6) putem specifičnih siRNA molekula. Učinkovitost utišavanja određena je 48 sati nakon transfekcije s siRNA protiv RPS6 u staničnim linijama A549, a testirane su tri konentracije kako bi se odredila ona koja najbolje sprječava izražaj ribosomskog proteina S6. Učinkovitost siRNA protiv RPS6 određena je upotrebom metode lančane reakcije polimeraze u stvarnom vremenu određivanjem izražaja gRNK za RPS6 te upotrebom metode Western blot određivanjem izražaj proteina RPS6. Dobiveni rezultati pokazuju da siRNA protiv RPS6, u koncentracijama 40 nM i 60 nM, vrlo uspješno dovodi do sniženja izražaja gRNK za RPS6 te posljedično smanjuje izražaj proteina RPS6. Stoga, testirana siRNA vrlo jer je učinkovita u sprječavanju izražaja RPS6 te se može koristiti u eksperimentalne svrhe za proučavanje funkcije ribosomskog proteina S6.
Abstract (english)
The study of genes and their functions are very important in order to gain insight into the molecular mechanisms that lead to diseases in humans. The use of small interfering RNAs, siRNAs, has great importance in studying the gene function and it is very successfully applied in genetic manipulation procedures. They are successfully used to prevent gene expression in the level of mRNA blocking its translation into protein. In this study, it was tested the efficiency of suppressing expression of ribosomal protein S6 (RPS6) with specific siRNA molecules. The efficiency of silencing was determined 48 hours after transfection with siRNA against RPS6 in A549 cell lines and three concentrations were tested to determine the one that best prevents expression of ribosomal protein S6. The efficiency of siRNA against RPS6 was determined by using the method polymerase chain reaction in real-time to measure the expression of mRNA for RPS6 and using the Western blot method to analyse the expression of RPS6 protein. The results show that siRNA against RPS6, at concentrations of 40 nM and 60 nM, very successfully leads to reduced expression of mRNA for RPS6 and consequently reduced expression of RPS6 protein. Therefore, the tested siRNA is highly effective in preventing expression of RPS6 and may be used for experimental purposes for the study of ribosomal protein S6 function.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:865056
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-12-06 18:10:24