prikaz prve stranice dokumenta Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica
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master's thesis
Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica
2018. urn:nbn:hr:184:691538
Vrbanović, Ana
University of Rijeka
Faculty of Medicine
Center for Proteomics

Cite this document

Vrbanović, A. (2018). Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:691538

Vrbanović, Ana. "Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:691538

Vrbanović, Ana. "Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:691538

Vrbanović, A. (2018). 'Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 15 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:691538

Vrbanović A. Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 November 15] Available at: https://urn.nsk.hr/urn:nbn:hr:184:691538

A. Vrbanović, "Protein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:691538

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Metadata
TitleProtein gp34/m04 mišjeg citomagalovirusa kao novi biljeg za detekciju virusom inficiranih stanica
Title (english)Murin citomegalovyrus protein gp34/m04 as a new marker for detection of virally infected cells
AuthorAna Vrbanović
MentorBerislav Lisnić (mentor)
MentorVanda Juranić Lisnić (mentor)
Committee memberAstrid Krmpotić (predsjednik povjerenstva)
Committee memberTihana Lenac Roviš (član povjerenstva)
Committee memberIgor Jurak (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Rijeka
Defense date and country2018-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj ovog rada bio je ispitati može li protutijelo m04.17, koje prepoznaje protein m04 mišjeg citomegalovirusa (MCMV-a), služiti kao marker infekcije stanica. Analiza specifičnosti vezanja protutijela anti-m04.17, proizvedenog u laboratoriju Centra za proteomiku, provedena je površinskim i unutarstaničnim obilježavanjem stanica inficiranih različitim sojevima MCMV-a 24 i 48 sati po infekciji. Protein m04 ispoljava se rano po infekciji te se nakuplja u stanicama tijekom infekcije u značajnim količinama. Prema dobivenim rezultatima, pokazalo se da je protutijelo m04.17 specifično obilježilo samo stanice zaražene virusom MCMV koji sadrži gen m04 (WT i Δm138). Osim toga, pokazalo se da proteina m04 ima mnogo više u unutrašnjosti stanice, nego na površini te da proteina m04 ima značajno više 48 sati po infekciji u usporedbi sa 24 sata po infekciji. Iz svega navedenog proizlazi da se protutijelo m04.17 može koristiti kao marker inficiranih stanica metodom koja će ga detektirati i u unutrašnjosti i na površini stanice.
Abstract (english)
The aim of this work was to investigate whether the antibody m04.17, which recognizes protein m04 of mouse cytomegalovirus (MCMV), can be used as a marker of MCMV-infected cells. Analysis of the binding specificity of anti-m04.17 antibody, produced in the laboratory Center for proteomics, was performed by surface and intracellular labeling of the cells infected with various MCMV strains 24 and 48 hours after infection. Protein m04 is expressed early after infection and accumulates in the cell during infection in significant quantities. In this work, I have shown that the antibody m04.17 specifically labels only the cells that were infected with MCMV containing the gene m04 (WT and Δm138). In addition, intracellular levels of protein m04 were significantly higher than the cell surface fraction. I could also observe increased staining with anti-m04 antibody in both intracellular and surface staining at 48 hPI in comparison with 24 hPI which is consistent with previously published data showing accumulation of m04 during the infection. In conclusion, the antibody m04 can be used as a marker of infected cells by fixing the cells and performing intracellular staining.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:691538
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-12-06 15:31:46