prikaz prve stranice dokumenta Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma
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master's thesis
Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma
2018. urn:nbn:hr:184:633799
Vučinić, Damir
University of Rijeka
Faculty of Medicine
Department of General Pathology and Pathological Anatomy

Cite this document

Vučinić, D. (2018). Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:633799

Vučinić, Damir. "Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:633799

Vučinić, Damir. "Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:633799

Vučinić, D. (2018). 'Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 06 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:633799

Vučinić D. Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 December 06] Available at: https://urn.nsk.hr/urn:nbn:hr:184:633799

D. Vučinić, "Povezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:633799

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Metadata
TitlePovezanost proteinske ekspresije matriks metaloproteinaza 1, 2 i 13 s histopatološkim značajkama nodularnog melanoma
Title (english)Correlation of matrix metalloproteinase 1, 2 and 13 protein expression with histopathology of nodular melanoma
AuthorDamir Vučinić
MentorGordana Zamolo (mentor)
Committee memberNives Jonjić (predsjednik povjerenstva)
Committee memberElvira Mustać (član povjerenstva)
Committee memberIngrid Belac-Lovasić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Rijeka
Defense date and country2018-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pathology
Abstract
Cilj: Imunohistokemijskim metodama ispitati ekspresiju matriks metaloproteinaza (MMP), MMP-1, MMP-2 i MMP-13, u nodularnom melanomu te odrediti postoji li poveznica aktivacije istih enzima s patohistološkim značajkama melanoma. Materijali i metode: Retrospektivno su analizirani podaci iz baze Zavoda za patologiju i patološku anatomiju, Medicinskog fakulteta u Rijeci. U ispitivanoj skupina korišteno je tkivo biopsija 51 pacijenta s potvrđenim nodularnim melanomom, dok su kontrolnu skupinu činili displastični nevusi. U parafinsku kocku su Tissue microarray (TMa) metodom postavljeni cilindri tkiva koji su kasnije bojeni imunohistokemijski. Procijenjena je ekspresija pojedine MMP u cilindrima ispitivane i kontrolne skupine. Rezultati: Analiza nije pokazala statistički značajnu povezanost u korelaciji godina pacijenata u trenutku dijagnoze s stupnjem po Breslowu (P= 0.269) kao ni u i korelaciji starosti s Clark stupnjem (P= 0.714). Usporedba Mann-Whitney U testom pokazala je statistički značajno (P<0.0001) veću ekspresiju MMP-1, MMP-2 i MMP-13 na membrani stanica nodularnog melanoma u odnosu na kontrolnu skupinu. Ispitan je odnos ekspresije navedenih enzima s stupnjem po Clarku i Breslowu, ali ni u jednom od ta dva slučaja nije pronađena statistički značajna razlika. Zaključak: Naši rezultati ukazali su na povećanu ekspresiju svih ispitivanih MMP kod nodularnog melanoma u odnosu na kontrolnu skupinu displastičnih nevusa. Ipak, nismo dokazali vezu između povećane ekspresije MMP i patohistoloških obilježja melanoma.
Abstract (english)
Aim: Investigate the expression of matrix metalloproteinases (MMP), MMP-1, MMP-2 and MMP-13 in nodular melanoma and determine whether the activation of the same enzymes correlates with pathohistological characteristics of melanoma. Materials and methods: Retrospectively, data were analyzed from the database of the Department of Pathology, Faculty of Medicine Rijeka. In order to evalute MMP expression, imunohistochemical analysis were performed on tissue microarrays of nodular melanoma tissue (51 samples) and displastic nevii tissue as control gruop. Results: The analysis did not found a statistically significant correlation between the age of patients at the time of diagnosis with Breslow depth level (P = 0.269); neither in the same correlation with Clark's degree (P = 0.714). Comparison performed by Mann-Whitney U test showed statistically significant (P <0.0001) higher expression of MMP-1, MMP-2 and MMP-13 on the membrane of nodular melanoma cells compared to the control group. The relationship between the expression of these enzymes with Clark and Breslow was investigated, but no statistically significant difference was found in either of these two cases. Conclusion: Our results confirmed an increased expression of all examined MMPs in nodular melanoma compared to the control group. However, we did not prove the link between increased expression of MMP and pathohistological features of nodular melanoma.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:633799
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-11-21 16:30:16