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master's thesis
ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS
2017. urn:nbn:hr:184:855829
Stojčević, Ivana
University of Rijeka
Faculty of Medicine
Department of Microbiology and Parasitology

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Stojčević, I. (2017). ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:855829

Stojčević, Ivana. "ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS." Master's thesis, University of Rijeka, Faculty of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:184:855829

Stojčević, Ivana. "ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS." Master's thesis, University of Rijeka, Faculty of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:184:855829

Stojčević, I. (2017). 'ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 11 April 2025, https://urn.nsk.hr/urn:nbn:hr:184:855829

Stojčević I. ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2017 [cited 2025 April 11] Available at: https://urn.nsk.hr/urn:nbn:hr:184:855829

I. Stojčević, "ČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:184:855829

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Metadata
TitleČIMBENICI VIRULENCIJE UROPATOGENIH IZOLATA ENTEROCOCCUS FAECALIS
AuthorIvana Stojčević
MentorIvana Gobin (mentor)
Committee memberDarinka Vučković (predsjednik povjerenstva)
Committee memberDijana Tomić Linšak (član povjerenstva)
Committee memberIvana Gobin (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Rijeka
Defense date and country2017-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Enterococcus faecalis bakterija je kojoj se nedavno počela pridavati pažnja zbog sve učestalijih što hospitalnih što drugih infekcija. Prirodno se nalaze u crijevima čovjeka i životinja. No kada dospiju na mjesto gdje nisu dio prirodne flore mogu izazavati upale. Najčešće govorimo o mokraćnom sustavu gdje kao posljedicu rasta i razmnožavanja uzrokuju urinoinfekt. u ovom smo radu ispitali čimbenike virulencije te osjetljivost na antibiotike enterokoka izoliranih iz urina. Od čimbenika... More virulencije ispitivani su enzimi proteaza, lipaza, želatinaza, katalaza; ispitivana je hidroliza eskulina, hemoliza, rezistencija na lizozim, pokretljivost, izvanstanična sluz i biofilm, te agregacija i osjetljivost na antibiotike. Nakon provedenog istraživanja dokazano je da su svi sojevi katalaza negativni, dok su svi eskulin pozitivni. U više od 80% izolata dokazana je γ-hemoliza. Od ostalih enzima proteazu producira 59% izolata, lipazu 95% i želatinazu 70%. Tendenciju stvaranja biofilma i izvanstanične sluzi posjeduje 73% bakterija. Na koncentraciju od 60 mg/ml lizozima osjetljivo je 35% bakterija, dok su na niže koncentracije lizozima (20, 40 mg/ml lizozima) svi izolati rezistentni. Za agregaciju možemo reći da 31% bakterija agregira u postotku većem od 90%. Od antibiotika važno je izdvojiti gentamicin za kojeg postoji rezistencija kod 23% izolata te na norfloksacin 20% izolata. E. faecalis posjeduju više čimbenika virulencije, između kojih bi istaknula produkciju izvanstanične sluzi i biofilma, te njihovu sposobnost razgradnje kolagena u tijelu pomoću enzima želatinaze Less
Abstract (english)
Enterococcus faecalis bacterium has recently begun to pay attention due to the more frequent hospital infection and other infections. They are naturally found in the intestinal flora of man and animal. But when they reach a place where they are not part of a natural flora can cause inflammation. Most commonly, we are talking about urinary system, which as a result of growth and reproduction cause urine infestation. In this paper, we investigated virulence factors and sensitivity to... More the antibiotics of enterococci isolated from urine. Virulence factors who were investigated are protease enzymes, lipases, gelatinases, catalases; hemolysis, lysozyme resistance, mobility, extracellular mucus and biofilm, aggregation and sensitivity to antibiotics. After the study has been shown, all catalase strains are negative, while they are all esculin positive. In more than 80% of isolates, γ-hemolysis has been proven. Of the other enzymes protease is produced by 59% isolates, 95% lipase and 70% gelatinase. The tendency to create biofilm and extracellular mucus has 73% bacteria. At concentrations of 60 mg / ml lysozyme, 35% bacteria are susceptible, while all isolates with lysozyme lower concetration (20, 40 mg/ml lysozyme) are resistant. For aggregation we can say that 31% of bacteria aggregate in percentage more than 90%. Of the antibiotics it is important to distinguish gentamicin with resistance to 23% of isolates and norfloxacin with 20% isolates. E. faecalis possesses several virulence factors, among which the most important are extracellular mucus production and biofilms production, and their ability to degrade collagen in the body by the gelatin enzyme Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:855829
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-02-15 10:02:22
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