prikaz prve stranice dokumenta Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays
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Scientific paper - Original scientific paper
Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays
Journal of Cellular Physiology, 232 (2017), 3; 463-476. https://doi.org/10.1002/jcp.25503
Blagojević Zagorac, Gordana; Mahmutefendić, Hana; Mačešić, Senka; Karleuša, Ljerka; Lučin, Pero

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Blagojević Zagorac, G., Mahmutefendić, H., Mačešić, S., Karleuša, Lj. & Lučin, P. (2017). Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays. Journal of Cellular Physiology, 232. (3), 463-476. doi: 10.1002/jcp.25503

Blagojević Zagorac, Gordana, et al. "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays." Journal of Cellular Physiology, vol. 232, no. 3, 2017, pp. 463-476. https://doi.org/10.1002/jcp.25503

Blagojević Zagorac, Gordana, Hana Mahmutefendić, Senka Mačešić, Ljerka Karleuša and Pero Lučin. "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays." Journal of Cellular Physiology 232, no. 3 (2017): 463-476. https://doi.org/10.1002/jcp.25503

Blagojević Zagorac, G., et al. (2017) 'Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays', Journal of Cellular Physiology, 232(3), pp. 463-476. doi: 10.1002/jcp.25503

Blagojević Zagorac G, Mahmutefendić H, Mačešić S, Karleuša Lj, Lučin P. Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays. Journal of Cellular Physiology [Internet]. 2017 [cited 2024 December 26];232(3):463-476. doi: 10.1002/jcp.25503

G. Blagojević Zagorac, H. Mahmutefendić, S. Mačešić, Lj. Karleuša and P. Lučin, "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays", Journal of Cellular Physiology, vol. 232, no. 3, pp. 463-476, 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:221896. [Accessed: 26 December 2024]

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Metadata
Title (english)Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays
AuthorGordana Blagojević Zagorac
AuthorHana Mahmutefendić
AuthorSenka Mačešić
AuthorLjerka Karleuša
AuthorPero Lučin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Abstract (english)
In this report, we present an analysis of several recycling protocols based on labeling of membrane proteins with specific monoclonal antibodies (mAbs). We analyzed recycling of membrane proteins that are internalized by clathrin-dependent endocytosis, represented by the transferrin receptor, and by clathrin-independent endocytosis, represented by the Major Histocompatibility Class I molecules. Cell surface membrane proteins were labeled with mAbs and recycling of mAb:protein complexes was determined by several approaches. Our study demonstrates that direct and indirect detection of recycled mAb:protein complexes at the cell surface underestimate the recycling pool, especially for clathrin-dependent membrane proteins that are rapidly reinternalized after recycling. Recycling protocols based on the capture of recycled mAb:protein complexes require the use of the Alexa Fluor 488 conjugated secondary antibodies or FITC-conjugated secondary antibodies in combination with inhibitors of endosomal acidification and degradation. Finally, protocols based on the capture of recycled proteins that are labeled with Alexa Fluor 488 conjugated primary antibodies and quenching of fluorescence by the anti-Alexa Fluor 488 displayed the same quantitative assessment of recycling as the antibody-capture protocols.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionAccepted version
Journal titleJournal of Cellular Physiology
Numberingvol. 232, no. 3, pp. 463-476
p-ISSN0021-9541
e-ISSN1097-4652
DOIhttps://doi.org/10.1002/jcp.25503
URN:NBNurn:nbn:hr:184:221896
Publication2017
ProjectNumber: HRZZ-IP-2014-09-9564
Title (croatian): Karakterizacija preuređivanja endosomalnih membranatijekom rane faze infekcije mišjim citomegalovirusom koje vodi k formiranju kompleksa za stvaranje viriona (Pero Lučin)
Jurisdiction: Croatia
Document URLhttp://bib.irb.hr/882266
Document URLhttp://onlinelibrary.wiley.com/doi/10.1002/jcp.25503/abstract
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2017-10-01 16:40:35