prikaz prve stranice dokumenta The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
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Scientific paper - Original scientific paper
The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
Polymers, 15 (2023), 12; 2663. https://doi.org/10.3390/polym15122663
Zdraveva, Emilija; Dolenec, Tamara; Tominac Trcin, Mirna; Govorčin Bajsić, Emi; Holjevac Grgurić, Tamara; Tomljenović, Antoneta; Dekaris, Iva; Jelić, Josip; Mijovic, Budimir

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Zdraveva, E., Dolenec, T., Tominac Trcin, M., Govorčin Bajsić, E., Holjevac Grgurić, T., Tomljenović, A. ... Mijovic, B. (2023). The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair. Polymers, 15. (12). doi: 10.3390/polym15122663

Zdraveva, Emilija, et al. "The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair." Polymers, vol. 15, no. 12, 2023. https://doi.org/10.3390/polym15122663

Zdraveva, Emilija, Tamara Dolenec, Mirna Tominac Trcin, Emi Govorčin Bajsić, Tamara Holjevac Grgurić, Antoneta Tomljenović, Iva Dekaris, Josip Jelić and Budimir Mijovic. "The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair." Polymers 15, no. 12 (2023). https://doi.org/10.3390/polym15122663

Zdraveva, E., et al. (2023) 'The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair', Polymers, 15(12). doi: 10.3390/polym15122663

Zdraveva E, Dolenec T, Tominac Trcin M, Govorčin Bajsić E, Holjevac Grgurić T, Tomljenović A, and sur.. The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair. Polymers [Internet]. 2023 June 13 [cited 2024 May 12];15(12). doi: 10.3390/polym15122663

E. Zdraveva, et al., "The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair", Polymers, vol. 15, no. 12, June 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:302114. [Accessed: 12 May 2024]

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Title (english)The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
AuthorEmilija Zdraveva
AuthorTamara Dolenec
AuthorMirna Tominac Trcin
AuthorEmi Govorčin Bajsić
AuthorTamara Holjevac Grgurić
AuthorAntoneta Tomljenović
AuthorIva Dekaris
AuthorJosip Jelić
AuthorBudimir Mijovic
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Ophthalmology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Ophthalmology
Abstract (english)
Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blockage of abnormal cornea vascularization. In terms of physicochemical properties, the biological component increased the PCL scaffold fiber diameter (by ~24%) and pore area (by ~82%), while ut slightly reduced its total porosity as the anti-VEGF solution filled the voids of the microfibrous structure. The addition of the anti-VEGF increased the scaffold stiffness almost three-fold at both strains of 5 and 10%, as well as its biodegradation rate (~36% after 60 days) with a sustained release profile after Day 4 of phosphate buffered saline incubation. In terms of scaffold application function, the PCL/Anti-VEGF scaffold proved to be more favorable for the adhesion of cultured limbal stem cells (LSCs); this was confirmed by the SEM images, where the cells showed flat and elongated conformations. Further support of the LSC growth and proliferation was confirmed by the identified p63 and CK3 markers after cell staining. These results demonstrate the advantageous effect of the surface-adsorbed anti-VEGF to stop vision loss and help damaged corneal tissue repair.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titlePolymers
Numberingvol. 15, no. 12, 2663
e-ISSN2073-4360
DOIhttps://doi.org/10.3390/polym15122663
URN:NBNurn:nbn:hr:184:302114
Publication2023-06-13
Document URLhttps://www.mdpi.com/2073-4360/15/12/2663
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-06-19 10:49:30