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Scientific paper - Original scientific paper
Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm
Hygiene, 3 (2023), 2; 125-135. https://doi.org/10.3390/hygiene3020011
Kovač, Bruno; Planinić, Ana; Planinić, Matej; Piletić, Kaća; Gobin, Ivana

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Kovač, B., Planinić, A., Planinić, M., Piletić, K. & Gobin, I. (2023). Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm. Hygiene, 3. (2), 125-135. doi: 10.3390/hygiene3020011

Kovač, Bruno, et al. "Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm." Hygiene, vol. 3, no. 2, 2023, pp. 125-135. https://doi.org/10.3390/hygiene3020011

Kovač, Bruno, Ana Planinić, Matej Planinić, Kaća Piletić and Ivana Gobin. "Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm." Hygiene 3, no. 2 (2023): 125-135. https://doi.org/10.3390/hygiene3020011

Kovač, B., et al. (2023) 'Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm', Hygiene, 3(2), pp. 125-135. doi: 10.3390/hygiene3020011

Kovač B, Planinić A, Planinić M, Piletić K, Gobin I. Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm. Hygiene [Internet]. 2023 April 17 [cited 2024 December 12];3(2):125-135. doi: 10.3390/hygiene3020011

B. Kovač, A. Planinić, M. Planinić, K. Piletić and I. Gobin, "Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm", Hygiene, vol. 3, no. 2, pp. 125-135, April 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:996890. [Accessed: 12 December 2024]

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Metadata
Title (english)Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm
AuthorBruno Kovač
AuthorAna Planinić
AuthorMatej Planinić
AuthorKaća Piletić
AuthorIvana Gobin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Ozone is a triatomic allotropic modification of oxygen with very high oxidation potential and strong antimicrobial properties, and can be used as a disinfecting agent. The aim of this work was to investigate the effectiveness of gaseous ozone in reducing the number of bacteria and the total biomass of E. coli biofilm using different methods of quantification and detection. Biofilm of all tested clinical isolates and standard strain was grown on ceramic tiles with dimensions of 1.0 × 1.0 cm over 24 h. These plates were then treated with gaseous ozone for 1 h. After washing, CFU/cm2 was determined, ATP bioluminescence was measured with a luminometer, and the total biomass reduction was measured after crystal-violet staining. Gaseous ozone proved to be very effective in destroying the created bacterial biofilm on ceramic tiles. Treatment caused a reduction in total bacteria number of up to 2.00 log10CFU/cm2, followed by a reduction in total biomass of up to 61.40%. Inhibition rates varied from 35.80% to 99.41%, depending on the method of quantification used. All methods used in this study are effective in determining the anti-biofilm activity of gaseous ozone, but more research is needed.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleHygiene
Numberingvol. 3, no. 2, pp. 125-135
e-ISSN2673-947X
DOIhttps://doi.org/10.3390/hygiene3020011
URN:NBNurn:nbn:hr:184:996890
Publication2023-04-17
Document URLhttps://www.mdpi.com/2673-947X/3/2/11
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-04-18 07:27:14