prikaz prve stranice dokumenta Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis
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Scientific paper - Original scientific paper
Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis
Rheumatology international, 32 (2012), 9; 2777-2784. https://doi.org/10.1007/s00296-011-2013-9
Massari, Dražen; Prpić-Massari, Larisa; Kehler, Tatjana; Kaštelan, Marija; Ćurkovic, Božidar; Peršić, Viktor; Ružić, Alen; Laškarin, Gordana

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Massari, D., Prpić Massari, L., Kehler, T., Kaštelan, M., Ćurkovic, B., Peršić, V. ... Laškarin, G. (2012). Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis. Rheumatology international, 32. (9), 2777-2784. doi: 10.1007/s00296-011-2013-9

Massari, Dražen, et al. "Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis." Rheumatology international, vol. 32, no. 9, 2012, pp. 2777-2784. https://doi.org/10.1007/s00296-011-2013-9

Massari, Dražen, Larisa Prpić Massari, Tatjana Kehler, Marija Kaštelan, Božidar Ćurkovic, Viktor Peršić, Alen Ružić and Gordana Laškarin. "Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis." Rheumatology international 32, no. 9 (2012): 2777-2784. https://doi.org/10.1007/s00296-011-2013-9

Massari, D., et al. (2012) 'Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis', Rheumatology international, 32(9), pp. 2777-2784. doi: 10.1007/s00296-011-2013-9

Massari D, Prpić Massari L, Kehler T, Kaštelan M, Ćurkovic B, Peršić V, and sur.. Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis. Rheumatology international [Internet]. 2012 [cited 2024 May 13];32(9):2777-2784. doi: 10.1007/s00296-011-2013-9

D. Massari, et al., "Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis", Rheumatology international, vol. 32, no. 9, pp. 2777-2784, 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:367270. [Accessed: 13 May 2024]

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Metadata
Title (english)Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis
AuthorDražen Massari
AuthorLarisa Prpić-Massari
AuthorTatjana Kehler
AuthorMarija Kaštelan
AuthorBožidar Ćurkovic
AuthorViktor Peršić
AuthorAlen Ružić
AuthorGordana Laškarin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Dermatovenerology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Internal Medicine)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Rehabilitatiom Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract (english)
The objective of the present study was to investigate possible changes in granulysin (GNLY)-mediated cytotoxicity of peripheral blood lymphocytes in psoriatic arthritis (PsA) patients with respect to different phases of the disease. We prospectively enrolled 25 PsA patients in the active phase, 26 PsA patients in remission and 24 healthy controls. The simultaneous detection of intracellular GNLY and cell surface antigens (CD3 and CD56) was performed with flow cytometry. GNLY apoptotic protein was visualised by immunocytochemistry. Natural killer (NK) cell cytotoxicity was analysed with a cytotoxicity assay against human erythroleukaemia K-562 cells. The percentage of GNLY(+) cells did not differ significantly between PsA patients in the acute phase and those in remission ; however, it was always higher than in healthy examinees due to the increased percentage of GNLY(+) cells within T cells, NKT cells, and both, and in the CD56(+dim) and CD56(+bright) NK subsets. The mean fluorescence intensity for GNLY was higher in all lymphocyte subpopulations in the acute phase than in remission and in healthy controls. Accordingly, GNLY-mediated NK cell cytotoxicity against K-562 cells of active phase PsA patients was significantly higher than that in patients in remission or in healthy controls. These findings demonstrated the involvement of GNLY in the worsening of PsA and suggested that GNLY mediated the development of joint lesions.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleRheumatology international
Numberingvol. 32, no. 9, pp. 2777-2784
p-ISSN0172-8172
DOIhttps://doi.org/10.1007/s00296-011-2013-9
URN:NBNurn:nbn:hr:184:367270
Publication2012
Document URLhttp://bib.irb.hr/534002
Type of resourceText
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2023-04-11 09:34:12