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undergraduate thesis
Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303
2022. urn:nbn:hr:184:262640
Radolović, Simone
University of Rijeka
Faculty of Medicine
Department of Chemistry and Biochemistry

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Radolović, S. (2022). Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303 (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:262640

Radolović, Simone. "Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:184:262640

Radolović, Simone. "Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:184:262640

Radolović, S. (2022). 'Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303', Undergraduate thesis, University of Rijeka, Faculty of Medicine, accessed 06 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:262640

Radolović S. Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303 [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2022 [cited 2024 December 06] Available at: https://urn.nsk.hr/urn:nbn:hr:184:262640

S. Radolović, "Analiza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303", Undergraduate thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:262640

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Metadata
TitleAnaliza proteoma mitohondrija kvasca Saccharomyces cerevisiae W303
Title (english)Mitochondria proteome analysis of the yeast Saccharomyces cerevisiae W303
AuthorSimone Radolović
MentorGordana Čanadi-Jurešić (mentor)
Committee memberBožena Ćurko-Cofek (predsjednik povjerenstva)
Committee memberSunčica Buljević (član povjerenstva)
Committee memberGordana Čanadi-Jurešić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Rijeka
Defense date and country2022-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Kvasci su pripadnici carstva gljiva (Fungi) kao i plijesni i mesnate gljive. Eukariotski su organizmi koji se često nazivaju jednostaničnim gljivama, a razmnožavaju se pupanjem. Saccharomyces cerevisiae neinfektivni je mikrob čiji je eukariotski genom bio prvi sekvencioniran. Svojim fenotipskim svojstvima poput učinkovitosti parenja ili sporulacije, doprinio je napretku u istraživanjima. Često korišteni modelni organizam upravo je soj Saccharomyces cerevisiae W303 koji je usko povezan s prvim potpuno sekvencioniranim sojem kvasca S288C te se stoga naziva njegovim potomkom. Mitohondriji, kao organeli većine eukariotskih organizama razlikuju se između različitih vrsta eukariota, po veličini, ali i sadržaju genoma. Cilj ovog rada je analizirati proteom mitohondrija kvasca Saccharomyces cerevisae W303. Stanice kvasca uzgajane su na kvaščevoj podlozi do ulaska u ranu stacionarnu fazu. Iz takvih su stanica izolirani mitohondriji, a iz njih proteini. Ekstrahirani proteini mitohondrija su nakon pripreme analizirani LC/MS metodom. Nakon identifikacije, dobiveni su proteini sistematizirani i utvrđena im je uloga i važnost u funkcioniranju stanice kvasca.
Abstract (english)
Yeasts are members of the fungi kingdom (Fungi), as well as molds and fleshy mushrooms. They are eukaryotic organisms often called unicellular fungi, and they reproduce by budding. Saccharomyces cerevisiae is a non-infectious microbe whose eukaryotic genome was the first to be sequenced. With their phenotypic properties such as mating efficiency or sporulation, they have contributed to scientific research progress. A frequently used model organism is the strain Saccharomyces cerevisiae W303, which is closely related to the first fully sequenced yeast strain S288C and is therefore called its descendant. Mitochondria, as organelles of most eukaryotic organisms, differ between different types of eukaryotes in terms of size and genome content. The aim of this work was to analyze the mitochondrial proteome of the yeast Saccharomyces cerevisae W303. Yeast cells were grown on yeast medium until they reached the early stationary phase. Mitochondria and proteins were isolated from such cells. After preparation, extracted mitochondrial proteins were analyzed by the LC/MS method. After identification, the obtained proteins were systematized and their role and importance in the functioning of the yeast cell was determined.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:262640
ProjectNumber: IPS-2020-01-7418
Title: Utvrđivanje pojavnosti, uzroka i štetnih učinaka oksidativnog stresa izazvanog uporabom fiksnih ortodontskih naprava
Acronym: IPS
Leader: Gordana Čanadi Jurešić
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: HRZZ-IPS
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2022-09-12 19:11:49