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doctoral thesis
IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE
2016. urn:nbn:hr:184:950328
Dominović, Marin
University of Rijeka
Faculty of Medicine
Department of Physiology, Immunology and Pathophxsiology

Cite this document

Dominović, M. (2016). IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE (Doctoral thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:950328

Dominović, Marin. "IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE." Doctoral thesis, University of Rijeka, Faculty of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:184:950328

Dominović, Marin. "IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE." Doctoral thesis, University of Rijeka, Faculty of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:184:950328

Dominović, M. (2016). 'IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE', Doctoral thesis, University of Rijeka, Faculty of Medicine, accessed 14 May 2024, https://urn.nsk.hr/urn:nbn:hr:184:950328

Dominović M. IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE [Doctoral thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2016 [cited 2024 May 14] Available at: https://urn.nsk.hr/urn:nbn:hr:184:950328

M. Dominović, "IZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE", Doctoral thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:184:950328

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Metadata
TitleIZRAŽAJ I FUNKCIJA GRANULIZINA I PERFORINA U DECIDUI PRVOG TROMJESEČJA TRUDNOĆE
Title (english)EXPRESSION AND FUNCTION OF GRANULYSIN AND PERFORIN IN THE FIRST TRIMESTER PREGNANCY DECIDUA
AuthorMarin Dominović
MentorDaniel Rukavina (mentor)
Committee memberNeda Smiljan Severinski (predsjednik povjerenstva)
Committee memberSabina Rabatić (član povjerenstva)
Committee memberAstrid Krmpotić (član povjerenstva)
Committee memberDaniel Rukavina (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Rijeka
Defense date and country2016-04-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Cilj istraživanja: Ispitali smo: (1) prisutnost granulizinskih oblika u decidualnim limfocitima i limfocitima periferne krvi, (2) stupanj kolokalizacije oblika granulizina s perforinom i biljegom degranulacije LAMP-1 nakon aktivacije stanica, (3) utjecaj molekula HLA-C i HLA-G, te IL-15, IL-2 i HSP70 na stupanj kolokalizacije, (4) utjecaj molekula HSP70 i PIBF na izražaj granulizina i perforina u decidualnim limfocitnim subpopulacijma, (5) mehanizam citotoksičnosti decidualnih limfocita (6) prisutnost perforina-2 na majčinoembrijskom spoju zdrave i patoloških trudnoća, (7) utjecaj interferona na izražaj gRNA za granulizin, perforin i perforin-2. Materijal i metode: Uzorke decidualnog tkiva i perifernu krv dobivali smo s Klinike za ginekologiju i porodništvo KBC-a Rijeka. Korištene metode su: imunofluorescencija, Western blot, test citotoksičnosti, RT-qPCR i imunohistokemija. Rezultati: Aktivacijom decidualnih limfocita dolazi do povišene skolokalizacije oblika granulizina od 9 kDa i perforina s LAMP-1. Molekula HLA-C u decidualnim limfocitima snižava kolokalizaciju oblika granulizina od 9 kDa s perforinom, dok molekula HLA-G potiče kolokalizaciju oblika od 15 kDa. IL-15 u decidualnim limfocitima smanjuje stupanj kolokalizacije oblika granulizina od 9 kDa s LAMP-1. Molekula HSP70 u decidualnim limfocitima potiče kolokalizaciju oba oblika granulizina s LAMP-1. Blokiranje protugranulizinskim i protuperforinskim protutijelima smanjuje nekrozu ciljnih stanica u testu citotoksičnosti. Perforin-2 je izražan u zdravoj trudnoći i patološkim trudnoćama, a IFN-γ povećava izražaj gRNA za perforin-2. Zaključak: Ovo istraživanje po prvi puta ukazuju na važnost kolokalizacije oblika granulizina i perforina kao čimbenika apoptotičkog djelovanja na majčino-embrijskom spoju.
Abstract (english)
Aim of the study: We investigated the: (1) expression of granulysin forms in decidual and peripheral blood lymphocytes, (2) colocalization of granulysin forms with perforin and LAMP-1 (marker of degranulation) after cell activation, (3) influence of HLA-C and HLAG molecules, and IL-15, IL-2 and HSP70 on colocalization, (4) influence of HSP70 and PIBF molecules on granulysin and perforin expression in decidual lymphocytes subpopulations, (5) cytotoxicity mechanism of decidual lymphocytes, (6) expression of perforin-2 in healthy and pathological pregnancies, (7) influence of interferons on mRNA expression for granulysin, perforin and perforin-2. Materials and methods: Decidual tissue and peripheral blood were obtained from Clinic of Gynecology and Obstetrics, Clinical Hospital Centre of Rijeka. We have used immunofluorescence, Western blotting, cytotoxicity assay, RT-qPCR and immunohistology. Results: After activation, 9 kDa granulysin form and perforin colocalize more with LAMP1. In decidual lymphocytes HLA-C molecule decreased the colocalization of 9 kDa granulysin with perforin, while HLA-G molecule increased the colocalization of 15 kDa granulysin with perforin. IL-15 in decidual lymphocytes decreased the colocalization of 9 kDa granulysin form with LAMP-1. HSP70 increased the colocalization of both granulysin forms with LAMP-1. By using using anti-granulysin and anti-perforin antibodies we have decreased the target cell necrosis. Perforin-2 is expressed in both healthy and pathological pregnancies, while IFN-γ upregulates the mRNA for perforin-2. Conclusion: This investigation for the first time suggests the importance of granulysin form and perforin colocalization, as a factor of cytotoxic and cytolytic activity at the maternal-embryonic interface.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:950328
ProjectNumber: 062-0620402-0376
Title: Citokini i citolitički mehanizmi tijekom rane trudnoće
Title: Cytokines and cytolytic mechanisms in early pregnancy
Leader: Daniel Rukavina
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
Study programmeTitle: Biomedicine Postgraduate (doctoral) study programme
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2022-03-17 14:13:47