prikaz prve stranice dokumenta ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM
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doctoral thesis
ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM
2016. urn:nbn:hr:184:571670
Popović, Branka
University of Rijeka
Faculty of Medicine
Department of Histology and Embryology

Cite this document

Popović, B. (2016). ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM (Doctoral thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:571670

Popović, Branka. "ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM." Doctoral thesis, University of Rijeka, Faculty of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:184:571670

Popović, Branka. "ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM." Doctoral thesis, University of Rijeka, Faculty of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:184:571670

Popović, B. (2016). 'ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM', Doctoral thesis, University of Rijeka, Faculty of Medicine, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:184:571670

Popović B. ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM [Doctoral thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2016 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:184:571670

B. Popović, "ULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM", Doctoral thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:184:571670

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Metadata
TitleULOGA INTERLEUKINA-33 U PRIROĐENOM I STEČENOM IMUNOLOŠKOM ODGOVORU NA INFEKCIJU MIŠJIM CITOMEGALOVIRUSOM
Title (english)THE ROLE OF INTERLEUKIN-33 IN INNATE AND ADAPTIVE IMMUNE RESPONSE TO MOUSE CYTOMEGALOVIRUS INFECTION
AuthorBranka Popović
MentorAstrid Krmpotić (mentor)
Committee memberIgor Jurak (predsjednik povjerenstva)
Committee memberNada Čikeš (član povjerenstva)
Committee memberStipan Jonjić (član povjerenstva)
Committee memberAstrid Krmpotić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Rijeka
Defense date and country2016-12-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Cilj istraživanja: IL-33 ima važnu ulogu u imunološkom odgovoru na neke patogene, što uključuje i imunoregulacijsku funkciju u spriječavanju oštećenja tkiva kao posljedice samog imunološkog odgovora. Aktivirani citotoksični limfociti ispoljavaju receptor za IL-33, ST2, čije signaliranje između ostalog dovodi do ekspanzije stanica i lučenja proupalnih citokina. S druge strane, Treg stanice konstitutivno ispoljavaju receptor ST2 koji im omogućuje brzo djelovanje i inhibiciju efektorskog odgovora. Cilj ovog istraživanja je ispitati utjecaj IL-33 na imunološki nadzor infekcije citomegalovirusom (CMV) i razvoj imunopatologije, napose značaj i ulogu glavnih efektorskih stanica te njihovu regulaciju putem Treg stanica. Materijali i metode: Korištenjem miševa kojima nedostaje ST2 (ST2-/- ) istražila sam in vivo učinak IL-33 u kontroli infekcije mišjim CMV-om. Funkcionalnim testovima i protočnom citometrijom analizirala sam fenotip i funkciju stanica NK, Treg i CD8+ limfocita T nakon infekcije. Kako bih analizirala imunopatologiju koristila sam imunohistokemijske metode, te odredila količinu jetrenih enzima u serumu miševa. Rezultati: Nedostatak ekspresije ST2 rezultira narušenim imunološkim odgovorom stanica NK i CD8+ limfocita T. Unatoč tome, ST2-/- miševi kontroliraju replikaciju MCMV-a jednako kao i kontrolne životinje, ali razvijaju imunopatologiju u jetri. Oštećenje jetre ST2-/- miševa prati smanjeno nakupljanje Treg stanica, koje su ključne za prevenciju imunopatologije. Zaključak: Ovaj rad pokazuje mehanizam aktivacije stanica NK i CD8+ limfocita T pomoću IL-33 tijekom infekcije MCMV-om. Također, po prvi puta pokazuje imunoregulacijsku ulogu Treg stanica u jetri miševa inficiranih MCMV-om. Treg stanice spriječavaju oštećenje tkiva jetre prilikom efektorskog odgovora limfocita T, a njihovo nakupljanje u jetri ovisi o IL-33.
Abstract (english)
Objectives: Previous studies demonstrated an important role of IL-33 in immunity against various pathogens, but also its immunoregulatory function aimed to prevent tissue damage caused by overwhelming immune response. Activated cytotoxic lymphocytes express IL-33 receptor ST2, which after ligation induces expansion and secretion of proinflammatory cytokines in these cells. On the other hand, Treg cells constitutively express high amounts of ST2, enabling them to respond fast and promoting their inhibition of effector immune response. The main goal of this study was to investigate the impact of IL-33 on immune surveillance and immunopathology during cytomegalovirus (CMV) infection, more specifically, impact on effector cells such as NK and CD8+ T cells but also regulatory Treg cells. Materials and Methods: In order to determine the role of IL-33 during infection with murine CMV (MCMV) in vivo, I used ST2-deficient mice (ST2-/- ). Using different functional assays and flow cytometry, I analyzed the phenotype and function of NK cells, CD8+ T cells and Treg cells. To investigate immunopathology, I used immunohistochemical methods and analyzed levels of liver enzymes. Results: ST2-deficiency results in abrogated NK and CD8+ T cell response. However, ST2-/- mice are still able to control MCMV replication similar to normal mice. However, ST2-/- mice develop immunopathology in the liver which correlates with decreased accumulation of Treg cells, essential in preventing immunopathology. Conclusion: This study shows IL-33-mediated activation of NK and CD8+ T cells during MCMV infection. For the first time, we show immunoregulatory role of Treg cells in the liver of MCMV infected mice. Treg cells are able to inhibit liver damage during effector T cell response and their accumulation in the liver depends on IL-33.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:571670
ProjectNumber: 062-0621261-1263
Title: Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Title: Molecular mechanisms of immune evasion by cytomegalovirus
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
ProjectNumber: 062-0621261-1268
Title: Uloga imunosubverzivnih citomegalovirusnih gena u latenciji
Title: The role of cytomegalovirus immunoevasion genes in latency
Leader: Astrid Krmpotić
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
Study programmeTitle: Biomedicine Postgraduate (doctoral) study programme
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2022-03-17 13:43:46