undergraduate thesis
Antimikrobni učinak propolisa na odabrane patogene streptokoke i kandidu

Davor Vidović (2016)
Sveučilište u Rijeci
Medicinski fakultet
Katedra za mikrobiologiju i parazitologiju
Metadata
TitleAntimikrobni učinak propolisa na odabrane patogene streptokoke i kandidu
AuthorDavor Vidović
Mentor(s)Ivana Gobin (thesis advisor)
Abstract
Propolis je ljepljiva tvar koju pčele radilice skupljaju s kore drveta, lisnih pupoljaka i cvjetova te ga obogaćuju enzimima. Biološka svojstva ovise o njegovom kemijskom sastavu koji se razlikuje ovisno od koje biljke i na kojem geografskom području je prikupljen. U ovom radu, propolis je ekstrahiran pomoću 70 % etilnog alkohola primjenom različitih metoda: metodom maceracije, izlaganjem ultrazvučnim valovima (15 min i 30 min) i izlaganjem mikrovalovima ( 2 x 10s i 3 x 10s). Štok otopine pet različitih uzoraka propolisa dobivenih različitim metodama ekstrakcije pripremljene su u koncentraciji od 25 mg / ml. Antimikrobni učinak testiran je na odabranim mikroorganizmima: bakterije iz roda Streptococcus (S. pyogenes, S. pneumoniae i S. salivarius) te gljivi Candida albicans. Antimikrobni učinak je ispitan metodom difuzije uz bušenje rupa u agaru i odreĎivanjem minimalne inhibicijske i baktericidne koncentracije. Kao rezultat metode difuzije, dobivene su zone inhibicije. Rezultati pokazuju da je najosjtljivija bakterija S. pneumoniae te zona inhibicije za ovu bakteriju iznosi 22 mm, a navedena zona se odnosi na ekstrakt dobiven metodom maceracije. Najlošija antimikrobna svojstva pokazuje uzorak propolisa dobiven metodom ekstrakcije s mikrovalovima u trajanju od 20 sekundi. Minimalna baktericidna koncentracija je najmanja za bakteriju S.salivarius i iznosi 0,4 mg/ml dok je najotpornija bakterija bila S. pyogenes za koju MBK iznosi 6,3 mg / ml. Rezultati testiranih bakterija i gljive pokazuju dobru osjetljivost na svih pet uzoraka propolisa te se on moţe koristiti kao pomoć pri liječenju infekcija uzrokovanih navedenim mikroorganizmima.
Keywordspropolis antimicrobial activity microorganisms of the oral cavity
Committee MembersDijana Tomić Linšak (committee chairperson)
Vanja Vasiljev Marchesi (committee member)
Ivana Gobin (committee member)
GranterSveučilište u Rijeci
Medicinski fakultet
Lower level organizational unitsKatedra za mikrobiologiju i parazitologiju
PlaceRijeka
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Study programme typeuniversity
Study levelundergraduate
Study programmeStudy of Sanitary Engineering
Academic title abbreviationuniv. bacc. sanit. ing.
Genreundergraduate thesis
Language Croatian
Defense date2016-07-13
Parallel abstract (English)
Propolis is a sticky substance that worker bees collect from the bark of trees, buds and flowers, and enriches it through enzymes. The biological properties depend on their chemical composition that varies depending on which plants and which geographical area it was collected from. In this research, propolis was extracted with 70% ethyl alcohol by using different methods: maceration method, exposure to ultrasound waves (15 min and 30 min) and exposure to microwaves (2 x 10s and 3 x 10s). Stock solutions of five different samples of propolis obtained by different extraction methods were prepared at a concentration of 25 mg / ml. The antimicrobial effect was tested on selected microorganisms: bacteria of the genus Streptococcus (S. pyogenes, S. pneumoniae and S. salivarius) and the fungus Candida albicans. The antimicrobial effect was tested through the method of diffusion by drilling holes in the agar and determining the minimum inhibitory and bactericidal concentrations. As a result of the diffusion method, zones of inhibition were obtained. The results show that the most sensitive bacteria was S. pneumoniae. The zone of inhibition for these bacteria was about 22 mm, with the extract obtained by maceration. The worst antimicrobial properties of propolis was exhibited by the sample obtained by the method of extraction with microwaves lasting 20 seconds. Minimum bactericidal concentration was least for the bacteria S. salivarius at 0.4 mg / ml while the most resistant bacteria was S. pyogenes with the MBC at 6.3 mg / ml. Results of the tested bacteria and fungi showed high sensitivity to all five samples of propolis, thus it can be used as an aid in the treatment of infections caused by tested microorganisms.
Parallel keywords (Croatian)propolis antimikrobno djelovanje mikroorganizmi usne šupljine
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:184:412705
CommitterBosa Licul