master's thesis
Antimikrobno djelovanje hidrolata smilja, ružmarina i lavandina na legionelu

Sanja Dobrić (2016)
Sveučilište u Rijeci
Medicinski fakultet
Katedra za mikrobiologiju i parazitologiju
Metadata
TitleAntimikrobno djelovanje hidrolata smilja, ružmarina i lavandina na legionelu
AuthorSanja Dobrić
Mentor(s)Ivana Gobin (thesis advisor)
Abstract
Legionella pneumophila je ubikvitarna bakterija koja najbolje preživljava u vodenim staništima poput jezera, rijeka, močvara i vlažnog tla. Nalazimo ju u bazenima, spa centrima, saunama i tuševima. Može uzrokovati legionarsku bolest i pontijačku groznicu ako se putem kontaminiranog aerosola unese u organizam. Osim uobičajenih tehnika prevencije legionele sve se više istražuju prirodne metode poput eteričnih ulja i njihovih produkata, hidrolata. Hidrolati su vodene mase koje zadržavaju slična molekulska svojstva kao i ulje, i za razliku od njih topiva su u vodi. Imaju jača antibakterijska, antivirusna, antigljivična i protuupalna svojstva, ali su nestabilni, lako se kvare ako se ne čuvaju pravilno. Cilj diplomskog rada je bio ispitati antimikrobno i antiagregativno djelovanje hidrolata smilja (Helichrysum italicum), ružmarina (Rosmarinus officinalis) i lavandina (Lavandula hybrida) na legionelu. Rezultati istraživanja pokazuju da je L. pneumophila osjetljiva na sve testirane hidrolate. Najbolju antibakterijsku aktivnost u bujonu pokazali su hidrolati smilja (Helicrysum italicum) i ružmarina (Rosmarinus officinalis), s MIK i MBK vrijednostima od 25%. Najbolju antibakterijsku aktivnost u vodi pokazao je hidrolat ružmarina (Rosmarinus officinalis), s MIK vrijednosti od 6,25%,. Najbolje agregacijsko djelovanje imao je hidrolat smilja (Helicrysum italicum). U sterilnoj vodi iz slavine je postotak agregacije iznosio 64 %, a u bujonu 64,8 %. Najbolje rezultate za kombinaciju hidrolata u bujonu na agregaciju ima kombinacija lavandina i ružmarina sa 73,52%, te možemo reći da ta dva hidrolata imaju sinergistički učinak. Svi testirani hidrolati imaju velik potencijal primjene u kontroli i suzbijanju legioneloze.
KeywordsLegionella pneumophila hydrolats antimicrobial activit
Committee MembersVanja Vasiljev Marchesi (committee chairperson)
Dijana Tomić Linšak (committee member)
Ivana Gobin (committee member)
GranterSveučilište u Rijeci
Medicinski fakultet
Lower level organizational unitsKatedra za mikrobiologiju i parazitologiju
PlaceRijeka
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Study programme typeuniversity
Study levelgraduate
Study programmeStudy of Sanitary Engineering
Academic title abbreviationmag. sanit. ing.
Genremaster's thesis
Language Croatian
Defense date2016-07-13
Parallel abstract (English)
Legionella pneumophila is a ubiquitous bacterium with affinity for aquatic habitats such as lakes, rivers, wetlands and moist soil. Legionella can be found in swimming pools, spas, saunas and showers. It can cause Legionnaires' disease and Pontiac fever if it get into the body through the contaminated aerosols. Except the usual techniques of prevention of Legionella there are natural methods such as essential oils and their products, like hydrolats. Hydrolats are floral waters that hold similar molecular properties like oil, and unlike them are soluble in water. They have strong antibacterial, antiviral, antifungal and anti-inflammatory properties, but they are unstable, they can easily spoil if they are not stored properly. The aim of the study was to investigate the antimicrobial and antiaggregative activity of immortelle hydrolat (Helichrysum italicum), rosemary hydrolat (Rosmarinus officinalis) and lavender hydrolat (Lavandula hybrida) to legionella. The results showed that L. pneumophila is sensitive to all tested hydrolats. The best antibacterial activity in broth showed immortelle hydrolat (Helicrysum italicum) and rosemary immortelle (Rosmarinus officinalis), with a MIC and MBC of 25%. The best antibacterial activity in water showed rosemary hydrolat (Rosmarinus officinalis), with a MIC of 6.25%. The best effect on the aggregation had immortelle hydrolat (Helicrysum italicum) and the percentage of aggregation in water was 64%, in the broth 64.8%. The best results on the aggregation for the combination hydrolat broth has a combination of lavender and rosemary hydrolat with 73.52%. All tested hydrolats have great potential applications in the control and prevention of legionellosis.
Parallel keywords (Croatian)Legionella pneumophila hidrolati antimikrobno djelovanje
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:184:161471
CommitterBosa Licul