prikaz prve stranice dokumenta Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid
  Download
PDF 1.86 MB
Scientific paper - Letter
Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid
ACS Chemical Biology, 16 (2021), 8; 1344-1349. https://doi.org/10.1021/acschembio.1c00422
Berni, Francesca; Kalfopoulou, Ermioni; Gimeno Cardells, Ana M.; Carboni, Filippo; van der Es, Daan; Romero-Saavedra, Felipe; Laverde, Diana; Miklić, Karmela; Malić, Suzana; Roviš, Tihana L.; Jonjić, Stipan; Ali, Sara; Overkleeft, Herman S.; Hokke, Cornelis H.; van Diepen, Angela; Adamo, Roberto; Jimenez-Barbero, Jesus; van der Marel, Gijsbert A.; Huebner, Johannes; Codée, Jeroen D. C. More authors...

Cite this document

Berni, F., Kalfopoulou, E., Gimeno Cardells, A. M., Carboni, F., van der Es, D., Romero Saavedra, F. ... Codée, J. D. C. (2021). Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid. ACS Chemical Biology, 16. (8), 1344-1349. doi: 10.1021/acschembio.1c00422

Berni, Francesca, et al. "Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid." ACS Chemical Biology, vol. 16, no. 8, 2021, pp. 1344-1349. https://doi.org/10.1021/acschembio.1c00422

Berni, Francesca, Ermioni Kalfopoulou, Ana M. Gimeno Cardells, Filippo Carboni, Daan van der Es, Felipe Romero Saavedra, Diana Laverde, et al. "Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid." ACS Chemical Biology 16, no. 8 (2021): 1344-1349. https://doi.org/10.1021/acschembio.1c00422

Berni, F., et al. (2021) 'Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid', ACS Chemical Biology, 16(8), pp. 1344-1349. doi: 10.1021/acschembio.1c00422

Berni F, Kalfopoulou E, Gimeno Cardells AM, Carboni F, van der Es D, Romero Saavedra F, and sur.. Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid. ACS Chemical Biology [Internet]. 2021 July 13 [cited 2024 July 15];16(8):1344-1349. doi: 10.1021/acschembio.1c00422

F. Berni, et al., "Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid", ACS Chemical Biology, vol. 16, no. 8, pp. 1344-1349, July 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:454657. [Accessed: 15 July 2024]

Please login to the repository to save this object to your list.
Metadata
Title (english)Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid
AuthorFrancesca Berni
AuthorErmioni Kalfopoulou
AuthorAna M. Gimeno Cardells
AuthorFilippo Carboni
AuthorDaan van der Es
AuthorFelipe Romero-Saavedra
AuthorDiana Laverde
AuthorKarmela Miklić
AuthorSuzana Malić
AuthorTihana L. Roviš
AuthorStipan Jonjić
AuthorSara Ali
AuthorHerman S. Overkleeft
AuthorCornelis H. Hokke
AuthorAngela van Diepen
AuthorRoberto Adamo
AuthorJesus Jimenez-Barbero
AuthorGijsbert A. van der Marel
AuthorJohannes Huebner
AuthorJeroen D. C. Codée
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR-analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.
Languageenglish
Publication typeScientific paper - Letter
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleACS Chemical Biology
Numberingvol. 16, no. 8, pp. 1344-1349
p-ISSN1554-8929
e-ISSN1554-8937
DOIhttps://doi.org/10.1021/acschembio.1c00422
URN:NBNurn:nbn:hr:184:454657
Publication2021-07-13
ProjectNumber: 675671
Title (english): A training network for the rational design of the next generation of well-defined glycoconjugate vaccines
Acronym: GLYCOVAX
Jurisdiction: eu
Funding stream: H2020
Document URLhttps://pubs.acs.org/doi/10.1021/acschembio.1c00422
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2021-12-30 13:06:00