prikaz prve stranice dokumenta Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency
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Scientific paper - Original scientific paper
Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency
Nature Communications, 11 (2020), 1; 6324. https://doi.org/10.1038/s41467-020-20031-4
Ouwendijk, Werner J. D.; Depledge, Daniel P.; Rajbhandari, Labchan; Lenac Roviš, Tihana; Jonjić, Stipan; Breuer, Judith; Venkatesan, Arun; Verjans, Georges M. G. M.; Sadaoka, Tomohiko

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Ouwendijk, W. J. D., Depledge, D. P., Rajbhandari, L., Lenac Roviš, T., Jonjić, S., Breuer, J. ... Sadaoka, T. (2020). Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nature Communications, 11. (1). doi: 10.1038/s41467-020-20031-4

Ouwendijk, Werner J. D., et al. "Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency." Nature Communications, vol. 11, no. 1, 2020. https://doi.org/10.1038/s41467-020-20031-4

Ouwendijk, Werner J. D., Daniel P. Depledge, Labchan Rajbhandari, Tihana Lenac Roviš, Stipan Jonjić, Judith Breuer, Arun Venkatesan, Georges M. G. M. Verjans and Tomohiko Sadaoka. "Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency." Nature Communications 11, no. 1 (2020). https://doi.org/10.1038/s41467-020-20031-4

Ouwendijk, W. J. D., et al. (2020) 'Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency', Nature Communications, 11(1). doi: 10.1038/s41467-020-20031-4

Ouwendijk WJD, Depledge DP, Rajbhandari L, Lenac Roviš T, Jonjić S, Breuer J, and sur.. Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nature Communications [Internet]. 2020 December 10 [cited 2024 December 13];11(1). doi: 10.1038/s41467-020-20031-4

W. J. D. Ouwendijk, et al., "Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency", Nature Communications, vol. 11, no. 1, December 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:287530. [Accessed: 13 December 2024]

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Metadata
Title (english)Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency
AuthorWerner J. D. Ouwendijk
AuthorDaniel P. Depledge
AuthorLabchan Rajbhandari
AuthorTihana Lenac Roviš
AuthorStipan Jonjić
AuthorJudith Breuer
AuthorArun Venkatesan
AuthorGeorges M. G. M. Verjans
AuthorTomohiko Sadaoka
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Abstract Varicella-zoster virus (VZV) establishes lifelong neuronal latency in most humans world-wide, reactivating in one-third to cause herpes zoster and occasionally chronic pain. How VZV establishes, maintains and reactivates from latency is largely unknown. VZV transcription during latency is restricted to the latency-associated transcript (VLT) and RNA 63 (encoding ORF63) in naturally VZV-infected human trigeminal ganglia (TG). While significantly more abundant, VLT levels positively correlated with RNA 63 suggesting co-regulated transcription during latency. Here, we identify VLT-ORF63 fusion transcripts and confirm VLT-ORF63, but not RNA 63, expression in human TG neurons. During in vitro latency, VLT is transcribed, whereas VLT-ORF63 expression is induced by reactivation stimuli. One isoform of VLT-ORF63, encoding a fusion protein combining VLT and ORF63 proteins, induces broad viral gene transcription. Collectively, our findings show that VZV expresses a unique set of VLT-ORF63 transcripts, potentially involved in the transition from latency to lytic VZV infection.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleNature Communications
Numberingvol. 11, no. 1, 6324
e-ISSN2041-1723
DOIhttps://doi.org/10.1038/s41467-020-20031-4
URN:NBNurn:nbn:hr:184:287530
Publication2020-12-10
ProjectNumber: KK.011.1.01.0006
Title (croatian): Jačanje kapaciteta CerVirVac-a za istraživanja u virusnoj imunologiji i vakcinologiji
Leader: Stipan Jonjić
Jurisdiction: eu
ProjectNumber: N/A
Title (croatian): Centar izvrsnosti za virusnu imunologiju i cjepiva
Title (english): Centre of Excellence for Research in Viral Immunology and the Development of New Vaccines
Acronym: CERVirVac
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZCI
Document URLhttp://www.nature.com/articles/s41467-020-20031-4
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-01-11 18:27:40