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master's thesis
Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC
2020. urn:nbn:hr:184:341038
Cuissot, Corentin Pavao Andre
University of Rijeka
Faculty of Medicine
Department of Physiology, Immunology and Pathophxsiology

Cite this document

Cuissot, C. P. A. (2020). Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:341038

Cuissot, Corentin Pavao Andre. "Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC." Master's thesis, University of Rijeka, Faculty of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:184:341038

Cuissot, Corentin Pavao Andre. "Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC." Master's thesis, University of Rijeka, Faculty of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:184:341038

Cuissot, C. P. A. (2020). 'Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:341038

Cuissot CPA. Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2020 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:184:341038

C. P. A. Cuissot, "Utjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:184:341038

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Metadata
TitleUtjecaj mišjeg citomegalovirusa na biologiju membranskih proteina i ispoljavanje staničnog prionskog proteina PrPC
Title (english)The effect of murine cytomegalovirus on membrane protein biology and expression of cellular prion protein PrPC
AuthorCorentin Pavao Andre Cuissot
MentorTihana Lenac Roviš (mentor)
Committee memberBerislav Lisnić (predsjednik povjerenstva)
Committee memberHana Mahmutefendić Lučin (član povjerenstva)
Committee memberIlija Brizić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Rijeka
Defense date and country2020-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract
Stanični prionski protein PrPC otkriven je u istraživanjima o uzroku prionopatija, koje karakterizira agregacija specifičnih proteina i posljedična neurodegeneracija. PrPC posjeduje dva mjesta za glikozilaciju i većina prionskog proteina izoliranog iz tkiva je diglikozilirana. Glikozilacija PrPC promovira transport PrPC do plazmatske membrane. S izuzetkom manje zastupljenih transmembranskih i cijepanih oblika, PrPC provodi veliku većinu životnog vijeka vezan za membranu putem GPI-sidra. Najveću količinu proteina PrPC nalazimo u imunosno privilegiranim mjestima, poput mozga, oka i placente te u imunosnom sustavu. Kako bi izbjegli nadzor imunosnog sustava domaćina, herpesvirusi su razvili raznolike mehanizme koji im omogućuju jedinstvenu sposobnost cjeloživotnog preživljavanja u inficiranim domaćinima u obliku latentne infekcije sa sposobnošću reaktivacije kad je imunosni sustav oslabljen. Dio mehanizama se temelji na sintezi proizvoda virusnih gena koji specifično zaustavljaju sintezu ili unutarstanični transport različitih efektorskih molekula imunosnog odgovora. Mišji citomegalovirus (MCMV), član obitelji obitelji herpesvirusa, dokazano mijenja reciklirajuće unutarstanične transportne puteve da bi smanjio ispoljavanje molekula bitnih za imunosnu reakciju na površini stanica. Svrha ovog rada bila je uspostaviti metodu za vizualizaciju i praćenje proteina PrPC ispoljenog na plazmatskoj membrani neuralnih stanica te uspostaviti metodu istovremenog praćenja četiri parametra u stanici putem konfokalne imunofluorescentne analize. Stanice neuroblastoma, N2A, bile su uzgojene, potom inficirane s virusom MCMV i zatim analizirane istovremeno na četiri parametara, putem četiri fluorofore, koje su bile marker za PrPC, jezgru, MCMV infekciju te stanični odjeljak, pri čemu je odabran AP1 pozitivan odjeljak. PrPC nije kolokalizirao s AP1 pozitvnim odjeljkom te vjerojatno MCMV ne utječe na njegov unutarstanični prijenos putem AP1 pozitivnog odjeljka.
Abstract (english)
Cellular prion protein (PrPC) was discovered during research into the causes of prionopathis characterized by aggregation of specific proteins and subsequent neurodegeneration. PrPC possesses two glycosylation sites, and most tissue-isolated prion proteins are diglycosylated. PrPC glycosylation stimulates the transport of PrPC to the plasma membrane. With the exception of the less common transmembrane and split forms, PrPC spends most of its lifetime bound to a membrane with a GPI anchor. PrPC is most expressed in immunoprivileged sites, such as the brain, eye, and placenta, and in the immune system. To avoid controlling the host’s immune system, herpesviruses have developed various mechanisms that allow them to survive in infected hosts in the form of a latent infection with the ability to reactivate when the immune system is weakened. Several mechanisms are based on the synthesis of products of viral genes that specifically stop the synthesis or intracellular transport of various effector molecules of nonspecific and specific immune response. MCMV has been shown to alter the recycling of intracellular transport pathways to reduce the expression of molecules important for the immune response at the cell surface. The purpose of this study was to establish a method for visualization and monitoring of PrPC protein expressed on the plasma membrane of neural cells and to establish a method for simultaneous monitoring of four parameters in the cell by confocal immunofluorescence analysis. Neuroblastoma cells N2A were cultured, then infected with MCMV virus, and then analyzed simultaneously for four parameters, using 4 fluorophores, which were a marker for PrPC, nucleus, MCMV infection, and AP1. PrPC did not colocalize with the AP1 positive compartment and probably MCMV does not affect its intracellular sorting via the AP1 positive compartment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:341038
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-01-11 08:20:05