prikaz prve stranice dokumenta Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila
  Download
PDF 2.84 MB
Scientific paper - Original scientific paper
Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila
International Journal of Molecular Sciences, 21 (2020), 15; 5367. https://doi.org/10.3390/ijms21155367
Lesar, Andrija; Mušković, Martina; Begić, Gabrijela; Lončarić, Martin; Tomić Linšak, Dijana; Malatesti, Nela; Gobin, Ivana

Cite this document

Lesar, A., Mušković, M., Begić, G., Lončarić, M., Tomić Linšak, D., Malatesti, N. & Gobin, I. (2020). Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila. International Journal of Molecular Sciences, 21. (15). doi: 10.3390/ijms21155367

Lesar, Andrija, et al. "Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila." International Journal of Molecular Sciences, vol. 21, no. 15, 2020. https://doi.org/10.3390/ijms21155367

Lesar, Andrija, Martina Mušković, Gabrijela Begić, Martin Lončarić, Dijana Tomić Linšak, Nela Malatesti and Ivana Gobin. "Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila." International Journal of Molecular Sciences 21, no. 15 (2020). https://doi.org/10.3390/ijms21155367

Lesar, A., et al. (2020) 'Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila', International Journal of Molecular Sciences, 21(15). doi: 10.3390/ijms21155367

Lesar A, Mušković M, Begić G, Lončarić M, Tomić Linšak D, Malatesti N, and sur.. Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila. International Journal of Molecular Sciences [Internet]. 2020 July 28 [cited 2024 November 11];21(15). doi: 10.3390/ijms21155367

A. Lesar, et al., "Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila", International Journal of Molecular Sciences, vol. 21, no. 15, July 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:510066. [Accessed: 11 November 2024]

Please login to the repository to save this object to your list.
Metadata
Title (english)Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila
AuthorAndrija Lesar
AuthorMartina Mušković
AuthorGabrijela Begić
AuthorMartin Lončarić
AuthorDijana Tomić Linšak
AuthorNela Malatesti
AuthorIvana Gobin
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Environmental Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract (english)
Legionella pneumophila is an environmental bacterium, an opportunistic premise plumbing pathogen that causes the Legionnaires’ disease. L. pneumophila presents a serious health hazard in building water systems, due to its high resistance to standard water disinfection methods. Our aim was to study the use of photodynamic inactivation (PDI) against Legionella. We investigated and compared the photobactericidal potential of five cationic dyes. We tested toluidine blue (TBO) and methylene blue (MB), and three 3-N-methylpyridylporphyrins, one tetra-cationic and two tri-cationic, one with a short (CH3) and the other with a long (C17H35) alkyl chain, against L. pneumophila in tap water and after irradiation with violet light. All tested dyes demonstrated a certain dark toxicity against L. pneumophila; porphyrins with lower minimal effective concentration (MEC) values than TBO and MB. Nanomolar MEC values, significantly lower than with TBO and MB, were obtained with all three porphyrins in PDI experiments, with amphiphilic porphyrin demonstrating the highest PDI activity. All tested dyes showed increasing PDI with longer irradiation (0–108 J/cm2), especially the two hydrophilic porphyrins. All three porphyrins caused significant changes in cell membrane permeability after irradiation and L. pneumophila, co-cultivated with Acanthamoeba castellanii after treatment with all three porphyrins and irradiation, did not recover in amoeba. We believe our results indicate the considerable potential of cationic porphyrins as effective anti-Legionella agents.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 21, no. 15, 5367
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms21155367
URN:NBNurn:nbn:hr:184:510066
Publication2020-07-28
Document URLhttps://www.mdpi.com/1422-0067/21/15/5367
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-09-28 15:02:36