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Scientific paper - Original scientific paper
MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine
Journal of Immunology, 133 (1984); 647-652. urn:nbn:hr:184:524920
Jonjić, Stipan; Koszinowski, Ulrich H.

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Jonjić, S. & Koszinowski, U. H. (1984). MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine. Journal of Immunology, 133., 647-652. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:524920

Jonjić, Stipan and Ulrich H. Koszinowski. "MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine." Journal of Immunology, vol. 133, 1984, pp. 647-652. https://urn.nsk.hr/urn:nbn:hr:184:524920

Jonjić, Stipan and Ulrich H. Koszinowski. "MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine." Journal of Immunology 133 (1984): 647-652. https://urn.nsk.hr/urn:nbn:hr:184:524920

Jonjić, S. and Koszinowski, U. H. (1984) 'MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine', Journal of Immunology, 133, pp. 647-652. Available at: https://urn.nsk.hr/urn:nbn:hr:184:524920 (Accessed 20 November 2024)

Jonjić S, Koszinowski UH. MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine. Journal of Immunology [Internet]. 1984 [cited 2024 November 20];133:647-652. Available at: https://urn.nsk.hr/urn:nbn:hr:184:524920

S. Jonjić and U. H. Koszinowski, "MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine", Journal of Immunology, vol. 133, pp. 647-652, 1984. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:524920. [Accessed: 20 November 2024]

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Metadata
Title (english)MONOCLONAL ANTIBODIES REACTIVE WITH SWINE LYMPHOCYTES I. Antibodies to Membrane Structures That Define the Cytolytic T Lymphocyte Subset in the Swine
AuthorStipan Jonjić
AuthorUlrich H. Koszinowski
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
A panel of monoclonal antibodies (mAb) with specificity for swine leukocytes was prepared by somatic cell hybridization with the use of spleen cells from mice immunized with swine thymocytes. The reactivity of two mAb (295/33 and 122/28). which both immunoprecipitated from the surface of swine leukocytes an antigen termed 5-L2 with an apparent m.w. of 33 to 35 kilodaltons under reducing and 65 to 70 kilodaltons under nonreducing conditions. was investigated in detail. These mAb were reactive in indirect immunofluorescence with 50 to 60% of thymocytes. 35% of peripheral blood lymphocytes. and 55% of E rosette-positive cells; they were nonreactive with bone marrow cells. Ig+ B cells. nonrosetting lymphocytes. granulocytes. and monocytes. In functional studies. the elimination of 5-L2+ cells partially reduced the proliferative response to concanavalin A and pokeweed mitogen but not to Staphylococcus aureus and lipopolysaccharide. The 5-L2- subset proliferated well to alloantigens. Both cytolytic T effector cells and precursor cells carried the antigen 5-L2 and could be depleted from heterogeneous cell populations by both antibodies in the presence of complement. These data suggest that the mAb 295/33 and 122/28 recognize a specific polypeptide present on the surface of swine cytolytic T cells. These antibodies will be useful in studies on the swine immune system.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Immunology
Numberingvol. 133, pp. 647-652
p-ISSN0022-1767
e-ISSN1550-6606
URN:NBNurn:nbn:hr:184:524920
Publication1984
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-03-28 14:43:25