prikaz prve stranice dokumenta A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
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Scientific paper - Original scientific paper
A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
Scientific Reports, 8 (2018); 6340. https://doi.org/10.1038/s41598-018-24724-1
Best, Ashley; Price, Christopher; Ožanič, Mateja; Šantić, Marina; Jones, Snake; Abu Kwaik, Yousef

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Best, A., Price, C., Ožanič, M., Šantić, M., Jones, S. & Abu Kwaik, Y. (2018). A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae. Scientific Reports, 8.. doi: 10.1038/s41598-018-24724-1

Best, Ashley, et al. "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae." Scientific Reports, vol. 8, 2018. https://doi.org/10.1038/s41598-018-24724-1

Best, Ashley, Christopher Price, Mateja Ožanič, Marina Šantić, Snake Jones and Yousef Abu Kwaik. "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae." Scientific Reports 8 (2018). https://doi.org/10.1038/s41598-018-24724-1

Best, A., et al. (2018) 'A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae', Scientific Reports, 8. doi: 10.1038/s41598-018-24724-1

Best A, Price C, Ožanič M, Šantić M, Jones S, Abu Kwaik Y. A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae. Scientific Reports [Internet]. 2018 April 20 [cited 2024 November 28];8. doi: 10.1038/s41598-018-24724-1

A. Best, C. Price, M. Ožanič, M. Šantić, S. Jones and Y. Abu Kwaik, "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae", Scientific Reports, vol. 8, April 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:122051. [Accessed: 28 November 2024]

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Metadata
Title (english)A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
AuthorAshley Best
AuthorChristopher Price
AuthorMateja Ožanič
AuthorMarina Šantić
AuthorSnake Jones
AuthorYousef Abu Kwaik
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Legionella pneumophila invades protozoa with an “accidental” ability to cause pneumonia upon transmission to humans. To support its nutrition during intracellular residence, L. pneumophila relies on host amino acids as the main source of carbon and energy to feed the TCA cycle. Despite the apparent lack of a requirement for glucose for L. pneumophila growth in vitro and intracellularly, the organism contains multiple amylases, which hydrolyze polysaccharides into glucose monomers. Here we describe one predicted putative amylase, LamB, which is uniquely present only in L. pneumophila and L. steigerwaltii among the ~60 species of Legionella. Our data show that LamB has a strong amylase activity, which is abolished upon substitutions of amino acids that are conserved in the catalytic pocket of amylases. Loss of LamB or expression of catalytically-inactive variants of LamB results in a severe growth defect of L. pneumophila in Acanthamoeba polyphaga and human monocytesderived macrophages. Importantly, the lamB null mutant is severely attenuated in intra-pulmonary proliferation in the mouse model and is defective in dissemination to the liver and spleen. Our data show an essential role for LamB in intracellular replication of L. pneumophila in amoeba and human macrophages and in virulence in vivo.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 8, 6340
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-018-24724-1
URN:NBNurn:nbn:hr:184:122051
Publication2018-04-20
ProjectNumber: IP-2016-06-9003
Title (croatian): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Acronym: FRANCYCLE
Leader: Marina Šantić
Jurisdiction: Croatia
Funding stream: IP
Document URLhttp://www.nature.com/articles/s41598-018-24724-1
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-02-02 17:50:50