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undergraduate thesis
UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU
2018. urn:nbn:hr:184:547675
Vragović, Martina
University of Rijeka
Faculty of Medicine
Department of Chemistry and Biochemistry

Cite this document

Vragović, M. (2018). UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:547675

Vragović, Martina. "UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:547675

Vragović, Martina. "UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:547675

Vragović, M. (2018). 'UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU', Undergraduate thesis, University of Rijeka, Faculty of Medicine, accessed 03 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:547675

Vragović M. UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 November 03] Available at: https://urn.nsk.hr/urn:nbn:hr:184:547675

M. Vragović, "UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU", Undergraduate thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:547675

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Metadata
TitleUTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU
Title (english)INFLUENCE OF pH ON DYNAMICS OF DRUG CARRIER BASED ON N-(2-HYDROXYPROPYL)METHACRYLAMIDE
AuthorMartina Vragović
MentorDamir Klepac (mentor)
Committee memberDijana Detel (predsjednik povjerenstva)
Committee memberMarin Tota (član povjerenstva)
Committee memberDamir Klepac (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Rijeka
Defense date and country2018-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
U ovom radu ispitano je otpuštanje nitroksilnih radikala koji se koriste za liječenje bolesti uzrokovanih oksidacijskim stresom sa polimernog nosača temeljenog na poli[N-(2-hidroksipropil)metakrilamidu] (poly(HPMA)). 4-okso-2,2,6,6-tetrametilpiperidin-1-oksil (TEMPONE) radikali vezani su na poly(HPMA) nosač preko razmaknice koja sadrži pH-osjetljivu hidrazonsku vezu. Priređeni hidrofilni polimer otopljen je u fosfatnom puferu pri različitim pH vrijednostima (7,4, 6,8, 6,0, 5,0). Hidrodinamički radijus molekula nosača u otopinama određen je metodom dinamičkog raspršenja svjetlosti (DLS). Pokazano je da su molekule poly(HPMA) u otopinama poprimile konformaciju statističkog klupka hidrodinamičkog radijusa 3,5 nm sa širokom razdiobom veličina. Oslobađanje nitroksilnih radikala sa poly(HPMA) nosača određivano je metodom elektronske spinske rezonancije (ESR). Utvrđeno je da se radikali brzo oslobađaju sa nosača pri uvjetima karakterističnim za endosome tumorskih stanica (pH = 5,0) dok pri fiziološkim uvjetima (pH = 7,4) radikali uglavnom ostaju vezani na nosaču. Na ovaj način postiže se kontrolirano otpuštanje radikala u tumoru te se izbjegavaju neželjeni učinci nitroksilnih radikala na ostale organe i tkiva.
Abstract (english)
In this thesis, the release of nitroxyl radicals used to treat diseases caused by oxidative stress from poly[N-(2-hydroxypropyl)methacrylamide] (poly(HPMA)) is investigated. 4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPONE) radicals are attached to a poly(HPMA) carrier by a spacer containing pH-sensitive hydrazone linkage. The prepared hydrophilic polymer is dissolved in phosphate buffer at various pH values (7.4, 6.8, 6.0, 5.0). The hydrodynamic radius of the carrier molecules in solutions was determined by the dynamic light scattering method (DLS). It has been shown that the poly(HPMA) molecules in the solutions obtained random coil conformation with the hydrodynamic radius of 3.5 nm and broad size distribution. The release of nitroxyl radicals from poly(HPMA) carrier was determined by the electron spin resonance (ESR) method. It was found that the radicals are quickly released from the carrier under conditions characteristic for the endosomal tumor cells (pH = 5.0) while at physiological conditions (pH = 7.4) the radicals remained largely bound to the carrier. In this way, controlled release of the radicals in the tumor can be achieved and the unwanted effects of nitroxyl radicals on other organs and tissues are avoided.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:547675
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-12-07 08:28:07