prikaz prve stranice dokumenta Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus
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Scientific paper - Original scientific paper
Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus
Journal of Experimental Medicine, 166 (1987), 3; 668-677. https://doi.org/10.1084/jem.166.3.668
Volkmer, Hansjürgen; Bertholet, Christine; Jonjić, Stipan; Wittek, Riccardo; Koszinowski, Ulrich H.

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Volkmer, H., Bertholet, C., Jonjić, S., Wittek, R. & Koszinowski, U. H. (1987). Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus. Journal of Experimental Medicine, 166. (3), 668-677. doi: 10.1084/jem.166.3.668

Volkmer, Hansjürgen, et al. "Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus." Journal of Experimental Medicine, vol. 166, no. 3, 1987, pp. 668-677. https://doi.org/10.1084/jem.166.3.668

Volkmer, Hansjürgen, Christine Bertholet, Stipan Jonjić, Riccardo Wittek and Ulrich H. Koszinowski. "Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus." Journal of Experimental Medicine 166, no. 3 (1987): 668-677. https://doi.org/10.1084/jem.166.3.668

Volkmer, H., et al. (1987) 'Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus', Journal of Experimental Medicine, 166(3), pp. 668-677. doi: 10.1084/jem.166.3.668

Volkmer H, Bertholet C, Jonjić S, Wittek R, Koszinowski UH. Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus. Journal of Experimental Medicine [Internet]. 1987 [cited 2024 April 26];166(3):668-677. doi: 10.1084/jem.166.3.668

H. Volkmer, C. Bertholet, S. Jonjić, R. Wittek and U. H. Koszinowski, "Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus", Journal of Experimental Medicine, vol. 166, no. 3, pp. 668-677, 1987. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:800512. [Accessed: 26 April 2024]

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Metadata
Title (english)Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus
AuthorHansjürgen Volkmer
AuthorChristine Bertholet
AuthorStipan Jonjić
AuthorRiccardo Wittek
AuthorUlrich H. Koszinowski
Author's institutionUniversity of Rijeka
Faculty of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The murine immediate-early (IE) protein pp89 is a nonstructural virus- encoded phosphoprotein residing in the nucleus of infected cells, where it acts as transcriptional activator. Frequency analysis has shown that in BALB/c mice the majority of virus-specific CTL recognize IE antigens. The present study was performed to assess whether pp89 causes membrane antigen expression detected by IE-specific CTL. Site-directed mutagenesis has been used to delete the introns from gene ieI, encoding pp89, for subsequent integration of the continuous coding sequence into the vaccinia virus genome. After infection with the vaccinia recombinant, the authentic pp89 was expressed in cells that became susceptible to lysis by an IE-specific CTL clone. Priming of mice with the vaccinia recombinant sensitized polyclonal CTL that recognized MCMV- infected cells and transfected cells expressing pp89. Thus, a herpesviral IE polypeptide with essential function in viral transcriptional regulation can also serve as a dominant antigen for the specific CTL response of the host.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Experimental Medicine
Numberingvol. 166, no. 3, pp. 668-677
p-ISSN0022-1007
e-ISSN1540-9538
DOIhttps://doi.org/10.1084/jem.166.3.668
URN:NBNurn:nbn:hr:184:800512
Publication1987
Document URLhttp://jem.rupress.org/content/166/3/668.long
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-29 21:51:30