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master's thesis
PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA
2017. urn:nbn:hr:184:390544
Vučetić, Vana
University of Rijeka
Faculty of Medicine
Department of Chemistry and Biochemistry

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Vučetić, V. (2017). PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:390544

Vučetić, Vana. "PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA." Master's thesis, University of Rijeka, Faculty of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:184:390544

Vučetić, Vana. "PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA." Master's thesis, University of Rijeka, Faculty of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:184:390544

Vučetić, V. (2017). 'PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:390544

Vučetić V. PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2017 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:184:390544

V. Vučetić, "PROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:184:390544

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Metadata
TitlePROMJENE IZRAŽAJA ČIMBENIKA PRIVLAČENJA MONOCITA -1 TIJEKOM RAZVOJA KOLITISA
Title (english)CHANGES IN THE EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN–1 DURING THE COLITIS DEVELOPMENT
AuthorVana Vučetić
MentorDijana Detel (mentor)
Committee memberJadranka Varljen (predsjednik povjerenstva)
Committee memberIvana Marić (član povjerenstva)
Committee memberDijana Detel (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Rijeka
Defense date and country2017-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
UVOD: Ulcerozni kolitis (UK) je kronična upalna imunološki posredovane bolesti obilježena ponavljajućim upalnim procesima koji rezultiraju oštećenjem i gubitkom funkcije crijevne sluznice. Etiologija razvoja upale nije u cijelosti poznata, no pokazano je da ključnu ulogu u razvoju ima abnormalni imunološki odgovor kod genetski predisponiranih osoba uz djelovanje i drugih čimbenika poput, okoliša i prehrane. Dipeptidil-peptidaza IV (DPP IV/CD26) je transmembranski glikoprotein tipa II, serinska proteaza koja svojim proteolitičkimo djelovanjem, ulogom u međustaničnoj komunikaciji i prijenosu signala u stanicu sudjeluje u modulaciji imunoloških mehanizama koji vode k razvoju UK. Čimbenik privlačenja monocita-1 (engl. Monocyte Chemoattractant Protein-1; MCP–1) je jedan od ključnih kemokina koji reguliraju migraciju i infiltraciju monocita, odnosno makrofaga, ima važnu ulogu u procesu angiogeneze te u razvoju malignog procesa. CILJ: Obzirom da još uvijek nije u potpunosti istraženo na koji način molekula DPP IV/CD26 utječe na razvoj i rezoluciju kolitisa cilj ovog rada bio je ispitati utjecaj ove molekule na ključni faktor u migraciji upalnih stanica, MCP-1 kemokin, uspostavom pokusnog modela DSS kolitisa kod divljeg tipa (C57BL/6) i CD26 deficijentnih miševa (CD26-/-). MATERIJALI I METODE: Animalni model kolitisa izazvan je, kod obje ispitivane skupine miševa, primjenom otopine natrijev dekstran sulfata (DSS) kroz sedam dana per os. Životinje su žrtvovane u tri vremenske točke (3, 7 i 20 dan) kako bi se pratio razvoj kolitisa te potvrdila uspostava kolitisa i oporavka sluznice debeloga crijeva. Procjena uspostave i intenzitet kolitisa pratila se na dnevnoj bazi temeljem pojavnosti i intenziteta sistemskih i lokalnih markera upalnog procesa te izračunom indeksa aktivnosti bolesti. Promjene izražaja MCP-1 pratile su se na proteinskoj razini u debelome crijevu i serumu te na transkripcijskoj razini u debelome crijevu. Transkripcijski izražaj MCP-1 određena qPCR metodom, a izražaj na proteinskoj razini ELISA metodom. REZULTATI: Izražaj MCP-1 na proteinskoj razini u debelome crijevu tijekom razvoja i u akutnoj fazi kolitisa značajno je veći u odnosu na kontrolnu skupinu u obje ispitivane skupine miševa. Međutim, porast u CD26-/- miševa je gotovo dvostruko veći u odnosu na C57BL/6 miševe. Na transkripcijskoj razini porast izražaja MCP-1 3. i 7. dana kolitisa je značajno veći u C57BL/6 miševa. ZAKLJUČAK:. Promjene izražaj MCP-1 na proteinskoj i transkripcijskoj razini u upalno promijenjenom tkivu debeloga crijeva u CD26-/- životinja ukazuju na ulogu DPP IV/CD26 u procesu aktivacije ovog kemokina te indirektno u aktivaciji upalnih stanica te ostalih čimbenika upale.
Abstract (english)
INTRODUCTION: Ulcerative colitis (UC) is a chronic immune mediated disease characterized by recurrent episodes of inflammation of the colon. Etiology has not yet been clarified but it is considered that disease develops as a result of inadequate immune response in a genetically susceptible host including the interaction of environmental and nutritional factors. Dipeptidyl-peptidase IV (DPP IV/CD26) is a type II transmembrane glycoprotein, serine protease that through proteolytic cleavage, role in interstitial communication, signal transmission is involved in modulation of immune response which leads to the development of. UC. Monocyte Chemoattractant Protein-1 (MCP-1) is one of the key chemokines that regulate the migration and infiltration of monocytes or macrophages. GOAL: Given that it is still not fully understand how DPP IV/CD26 molecules influence on the development and resolution of colitis, the aim of this study was to examine the influence of this molecule on the main factor in the migration of inflammatory cells, MCP-1 chemokines by establishing experimental model of DSS colitis in wild type (C57BL/6) and CD26 deficient (CD26-/-) MATERIALS AND METHODS: Colitis was induced in both mouse strains using sodium dextran sulfate solution (DSS) administered for 7 days in drinking water ad libitum. In order to monitor the development of colitis and recovery both groups of animals were sacrificed on days 2, 7, and 20 following treatment. Evaluation of colitis establishment was monitored on a daily basis based on the appearance and intensity of inflammatory markers and calculation of disease activity index. Transcriptional activity of MCP-1 was done by qPCR, while protein expression was assessed by ELISA. RESULTS: Protein expression of colon MCP-1 during the development of colitis and in acute phase significantly increases in both mouse strains. However, in CD26-/- animals the increase of MCP-1 was twice that in C57BL/6 animals. The transcriptional activity of MCP-1 during colitis development and in the acute phase was higher in C57BL/6 animals. CONCLUSION: Changes of MCP-1 expression, at protein and transcriptional level, in inflamed colon tissue in CD26-/- animals implicate the involvement of DPP IV/CD26 in the process of activation of MCP-1 chemokine and indirectly in the activation of inflammatory cells and other factors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:390544
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-06-18 15:35:04