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Scientific paper - Original scientific paper
Antimycobacterial potential of the juniper berry essential oil in tap water
Arhiv za higijenu rada i toksikologiju, 69 (2018), 1; 46-53. https://doi.org/10.2478/aiht-2018-69-3085
Peruč, Dolores; Gobin, Ivana; Abram, Maja; Broznić, Dalibor; Svalina, Tomislav; Štifter, Sanja; Malenica Staver, Mladenka; Tićac, Brigita

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Peruč, D., Gobin, I., Abram, M., Broznić, D., Svalina, T., Štifter, S. ... Tićac, B. (2018). Antimycobacterial potential of the juniper berry essential oil in tap water. Arhiv za higijenu rada i toksikologiju, 69. (1), 46-53. doi: 10.2478/aiht-2018-69-3085

Peruč, Dolores, et al. "Antimycobacterial potential of the juniper berry essential oil in tap water." Arhiv za higijenu rada i toksikologiju, vol. 69, no. 1, 2018, pp. 46-53. https://doi.org/10.2478/aiht-2018-69-3085

Peruč, Dolores, Ivana Gobin, Maja Abram, Dalibor Broznić, Tomislav Svalina, Sanja Štifter, Mladenka Malenica Staver and Brigita Tićac. "Antimycobacterial potential of the juniper berry essential oil in tap water." Arhiv za higijenu rada i toksikologiju 69, no. 1 (2018): 46-53. https://doi.org/10.2478/aiht-2018-69-3085

Peruč, D., et al. (2018) 'Antimycobacterial potential of the juniper berry essential oil in tap water', Arhiv za higijenu rada i toksikologiju, 69(1), pp. 46-53. doi: 10.2478/aiht-2018-69-3085

Peruč D, Gobin I, Abram M, Broznić D, Svalina T, Štifter S, and sur.. Antimycobacterial potential of the juniper berry essential oil in tap water. Arhiv za higijenu rada i toksikologiju [Internet]. 2018 March 20 [cited 2024 November 13];69(1):46-53. doi: 10.2478/aiht-2018-69-3085

D. Peruč, et al., "Antimycobacterial potential of the juniper berry essential oil in tap water", Arhiv za higijenu rada i toksikologiju, vol. 69, no. 1, pp. 46-53, March 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:393436. [Accessed: 13 November 2024]

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Title (english)Antimycobacterial potential of the juniper berry essential oil in tap water
Title (croatian)Antimikobakterijski potencijal eteričnog ulja plodova borovice (Juniperus communis) u pitkoj vodi
AuthorDolores Peruč
AuthorIvana Gobin
AuthorMaja Abram
AuthorDalibor Broznić
AuthorTomislav Svalina
AuthorSanja Štifter
AuthorMladenka Malenica Staver
AuthorBrigita Tićac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Mycobacterium avium complex-related diseases are often associated with poorly maintained hot water systems. This calls for the development of new control strategies. The aim of this study was to investigate the activity of essential oils (EOs) from the Mediterranean plants, common juniper, immortelle, sage, lavandin, laurel, and white cedar against Mycobacterium avium ssp. avium, Mycobacterium intracellulare, and Mycobacterium gordonae in culturing broth and freshwater as their most common habitat. To do that, we developed a new method of water microdilution to determine their minimal effective concentrations (MEC). The most active EO was the one from the common juniper with the MEC of 1.6 mg mL-1. Gas chromatography / mass spectrometry the juniper EO identified monoterpenes (70.54 %) and sesquiterpenes (25.9 %) as dominant component groups. The main monoterpene hydrocarbons were α-pinene, sabinene, and β-pinene. The juniper EO significantly reduced the cell viability of M. intracellulare and M. gordonae at MEC, and of M. avium at 2xMEC. Microscopic analysis confirmed its inhibitory effect by revealing significant morphological changes in the cell membrane and cytoplasm of all three bacteria. The mode of action of the juniper EO on the cell membrane was confirmed by a marked leakage of intracellular material. Juniper EO has a great practical potential as a complementary or alternative water disinfectant in hot water systems such as baths, swimming pools, spa pools, hot tubs, or even foot baths/whirlpools.
Abstract (croatian)
Ispitali smo aktivnost eteričnih ulja borovice [Juniperus communis (J. communis)], smilja (Helichrysum italicum), kadulje (Salvia officinalis), lavandina (Lavandula hybrida), lovora (Laurus nobilis) i tuje (Thuja occidentalis) prema sojevima Mycobacterium avium ssp. avium (M. avium), Mycobacterium intracellulare (M. intracellulare) i Mycobacterium gordonae (M. gordonae). Za određivanje minimalnih inhibicijskih koncentracija (MIK) i minimalnih baktericidnih koncentracija (MBK) testiranih eteričnih ulja koristili smo se metodom mikrodiluacije u tekućem bujonu (Middlebrook 7H9), a za određivanje minimalne efektivne koncentracije (MEK) umjesto bujona korištena je sterilna voda iz slavine kojoj je dodano 0,05 % Tweena 80. Dvostruka serijska razrjeđenja eteričnih ulja, počevši od 0,1 do 51,2 mg mL-1, u tekućem bujonu ili u sterilnoj vodi iz slavine inokulirana su s mikobakterijskom suspenzijom uz dodatak resazurina. Najučinkovitije eterično ulje prema svim ispitivanim sojevima mikobakterija bilo je eterično ulje J. communis s MBK / MIK / MEK vrijednošću od 1,6 mg mL-1. Metodom plinske kromatografije i masene spektrometrije analizirano je eterično ulje J. communis. Udio monoterpena iznosio je 70,54 %, a seskviterpena 25,9 %. Glavni monoterpenski ugljikovodici bili su α-pinen, sabinen i β-pinen. Eterično ulje J. communis pokazalo je značajan inhibicijski učinak na M. intracellulare i M. gordonae pri MEK i na M. avium pri dvostrukim MEK. Elektronskom mikroskopijom kod svih triju sojeva mikobakterija nakon izlaganja djelovanju eteričnog ulja J. communis otkrivene su značajne morfološke promjene stanične membrane i citoplazme. Učinak eteričnog ulja J. communis na destrukciju stanične membrane mikobakterija potvrđen je značajnim otpuštanjem unutarstaničnog materijala mjerenjem apsorbancije supernatanta pri 260 nm i 280 nm. Zaključno, u našem smo radu razvili novu metodu za ispitivanje antimikrobnog učinka eteričnih ulja ili drugih prirodnih tvari na netuberkulozne mikobakterije koja oponaša uvjete kao u vodenim sustavima. Prirodni proizvodi, osobito eterična ulja, imaju ne samo velik potencijal kao antimikrobni agensi nego i moguću praktičnu primjenu kao alternativni dezinficijensi.
Keywords (english)
Keywords (croatian)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleArhiv za higijenu rada i toksikologiju
Numberingvol. 69, no. 1, pp. 46-53
p-ISSN0004-1254
e-ISSN1848-6312
DOIhttps://doi.org/10.2478/aiht-2018-69-3085
URN:NBNurn:nbn:hr:184:393436
Publication2018-03-20
Document URLhttps://hrcak.srce.hr/196023
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-03-21 17:51:24