Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats

Grubić Kezele, Tanja; Blagojević Zagorac, Gordana; Jakovac, Hrvoje; Domitrović, Robert; Radošević-Stašić, Biserka

doi:10.14670/HH-11-780

prikaz prve stranice dokumenta Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats

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Scientific paper - Original scientific paper

Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats

Histology and Histopathology, 32 (2017); 137-151. https://doi.org/10.14670/HH-11-780

Grubić Kezele, Tanja; Blagojević Zagorac, Gordana; Jakovac, Hrvoje; Domitrović, Robert; Radošević-Stašić, Biserka

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APA 6th Edition

Grubić Kezele, T., Blagojević Zagorac, G., Jakovac, H., Domitrović, R. & Radošević Stašić, B. (2017). Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats. Histology and Histopathology, 32., 137-151. doi: 10.14670/HH-11-780

MLA 8th Edition

Grubić Kezele, Tanja, et al. "Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats." Histology and Histopathology, vol. 32, 2017, pp. 137-151. https://doi.org/10.14670/HH-11-780

Chicago 17th Edition

Grubić Kezele, Tanja, Gordana Blagojević Zagorac, Hrvoje Jakovac, Robert Domitrović and Biserka Radošević Stašić. "Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats." Histology and Histopathology 32 (2017): 137-151. https://doi.org/10.14670/HH-11-780

Harvard

Grubić Kezele, T., et al. (2017) 'Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats', Histology and Histopathology, 32, pp. 137-151. doi: 10.14670/HH-11-780

Vancouver

Grubić Kezele T, Blagojević Zagorac G, Jakovac H, Domitrović R, Radošević Stašić B. Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats. Histology and Histopathology [Internet]. 2017 [cited 2024 November 15];32:137-151. doi: 10.14670/HH-11-780

IEEE

T. Grubić Kezele, G. Blagojević Zagorac, H. Jakovac, R. Domitrović and B. Radošević Stašić, "Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats", Histology and Histopathology, vol. 32, pp. 137-151, 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:466957. [Accessed: 15 November 2024]

Cite this item: https://urn.nsk.hr/urn:nbn:hr:184:466957

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Title (english)	Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats
Author	Tanja Grubić Kezele
Author	Gordana Blagojević Zagorac
Author	Hrvoje Jakovac
Author	Robert Domitrović
Author	Biserka Radošević-Stašić
Author's institution	University of Rijeka Faculty of Medicine (Department of Physiology, Immunology and Pathophxsiology)
Author's institution	University of Rijeka Faculty of Medicine (Department of Chemistry and Biochemistry)
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences
Abstract (english)	Inflammatory demyelinating diseases such as multiple sclerosis and experimental autoimmune encephalomyelitis (EAE) are often followed by cognitive deficits associated with the neuronal injury, synaptic loss and altered neurogenesis within the hippocampus. Changes depend on the genetic and epigenetic factors that ensure the cellular and environmental homeostasis and regulate the interactions of immunocompetent, glial and neural cells. Owing to high impact of stress proteins on these processes, in this study we compared the protein content of interleukin-6, transforming growth factor-β1, metallothioneins I/II (MTs) and glycoprotein 96 (gp96) in the hippocampus of DA and AO rats that differ in the susceptibility to the induction of EAE, and tested the relationship of MTs and gp96 to granule neurons, glial cells and neural progenitors in different subfields of dentate gyrus. Rats were immunized with bovine brain homogenate emulsified in complete Freund's adjuvant or only with CFA. The data showed that acute attack of EAE in DA rats was followed by accumulation of IL-6, TGF-β1 and MTs proteins, by increased expression of MTs in molecular and granular cell layer, by reduced expression of gp96/granular cell, by apoptosis and by microgliosis with appearance of Iba-1+ cells, co-expressing MT I/II and gp96. Furthermore, in subgranular zone (SGZ) of DA rats an augmented number of GFAP+ precursors, but decreased number of doublecortin (DCX)+ neuroblasts and immature NeuN+ neurons were found, implying that in DA rats the neurogenesis was delayed or reduced. Besides, in SGZ of both strains several DCX+ and NeuN+ cells co-expressing gp96 and MT I/II were found.
Keywords (english)
Language	english
Publication type	Scientific paper - Original scientific paper
Publication status	Published
Peer review	Peer review - international
Publication version	Published version
Journal title	Histology and Histopathology
Numbering	vol. 32, pp. 137-151
p-ISSN	0213-3911
e-ISSN	1699-5848
DOI	https://doi.org/10.14670/HH-11-780
URN:NBN	urn:nbn:hr:184:466957
Publication	2017
Document URL	https://www.ncbi.nlm.nih.gov/pubmed/27173051?dopt=Citation
Type of resource	Text
Access conditions	Closed access
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Created on	2017-09-21 07:39:59