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Scientific paper - Original scientific paper
Antimikrobni učinak Apexita in vitro
Acta stomatologica Croatica, 35 (2001), 4; 475-477. urn:nbn:hr:184:501904
Pezelj-Ribarić, Sonja; Brekalo, Ivana; Miletić, Ivana; Abram, Maja; Karlović, Zoran; Anić, Ivica

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Pezelj Ribarić, S., Brekalo, I., Miletić, I., Abram, M., Karlović, Z. & Anić, I. (2001). Antimikrobni učinak Apexita in vitro. Acta stomatologica Croatica, 35. (4), 475-477. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:501904

Pezelj Ribarić, Sonja, et al. "Antimikrobni učinak Apexita in vitro." Acta stomatologica Croatica, vol. 35, no. 4, 2001, pp. 475-477. https://urn.nsk.hr/urn:nbn:hr:184:501904

Pezelj Ribarić, Sonja, Ivana Brekalo, Ivana Miletić, Maja Abram, Zoran Karlović and Ivica Anić. "Antimikrobni učinak Apexita in vitro." Acta stomatologica Croatica 35, no. 4 (2001): 475-477. https://urn.nsk.hr/urn:nbn:hr:184:501904

Pezelj Ribarić, S., et al. (2001) 'Antimikrobni učinak Apexita in vitro', Acta stomatologica Croatica, 35(4), pp. 475-477. Available at: https://urn.nsk.hr/urn:nbn:hr:184:501904 (Accessed 14 December 2024)

Pezelj Ribarić S, Brekalo I, Miletić I, Abram M, Karlović Z, Anić I. Antimikrobni učinak Apexita in vitro. Acta stomatologica Croatica [Internet]. 2001 December 15 [cited 2024 December 14];35(4):475-477. Available at: https://urn.nsk.hr/urn:nbn:hr:184:501904

S. Pezelj Ribarić, I. Brekalo, I. Miletić, M. Abram, Z. Karlović and I. Anić, "Antimikrobni učinak Apexita in vitro", Acta stomatologica Croatica, vol. 35, no. 4, pp. 475-477, December 2001. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:501904. [Accessed: 14 December 2024]

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Metadata
Title (croatian)Antimikrobni učinak Apexita in vitro
Title (english)Antibacterial Activity of Calcium Hydroxide Root Canal Sealer (Apexit) - in vitro Study
AuthorSonja Pezelj-Ribarić
AuthorIvana Brekalo
AuthorIvana Miletić
AuthorMaja Abram
AuthorZoran Karlović
AuthorIvica Anić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Endodontics and Restorative Dentistry)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Endodonics and Restorative Dentistry
Abstract (croatian)
Svrha ovog rada bila je odrediti in vitro antimikrobni učinak Apexita temeljenog na kalcijevu hidroksidu u neposrednom dodiru s bakterijama: Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens i Staphylococcus aureus. Rabljen je Direct contact test (DCT) pri čemu je 10 μl bakterijske suspenzije (106 bakterija) ostavljeno u neposrednom dodiru s punilom 1 sat na 37°C. Pošto je dodan BHI medij (250 μl), mjeren je rast pojedine bakterije nakon 1 sat, 6 sati, 20 sati i 24 sata. Rezultati nakon jednog sata pokazali su da se broj E. coli i P. aeruginosa smanjio na 7-9 x 10 3, S. marcescens na 7 x 10 2 bakterija, a broj S. aureus bio je 1,4 x 10 5. cestosatni uzorci pokazali su da se broj P. aeruginosa smanjio na 10 1, a gram-pozitivne S. aureus na 7,5 x 10 4. Nakon 20 sati preživjela je jedino S. aureus (10 1). Dvadesetčetirisatni uzorci pokazali su potpunu inhibiciju rasta svih ispitivanih vrsta.
Abstract (english)
The aim of this study was to determine the in vitro antimicrobial activity of calcium hydroxide root canal sealer (Apexit) in direct contact with Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens and Staphylococcus aureus. The direct contact test (DCT) was performed. The sealer was mixed and placed on the side wall of microtiter plate wells and 10 μl of bacterial suspension (106 bacteria) was placed onto its surface. Bacteria were in direct contact with the sealer for 1 hour at 37°C. BHI broth (250 μl) was then added and the growth of each strain was measured after 1 hour, 6 hours, 20 hours and 24 hours. After 1 hour the number of E. coli and P. aeruginosa decreased to 7-9 x 10 3 and S. marcescens to 7 x 10 2 bacteria. The number of S. aureus was 1.4 x 10 5 bacteria. Six hour samples showed that the number of P. aeruginosa decreased to 10 1 and gram-positive S. aureus to 7.5 x 10 4. After 20 hours only S. aureus (10 1 bacteria) survived after prior contact with Apexit. The 24-hour samples showed complete bacterial growth inhibition of all the bacterial strains tested in DCT with Apexit.
Keywords (croatian)
Keywords (english)
Languagecroatian
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleActa stomatologica Croatica
Numberingvol. 35, no. 4, pp. 475-477
p-ISSN0001-7019
e-ISSN1846-0410
URN:NBNurn:nbn:hr:184:501904
Publication2001-12-15
Document URLhttp://hrcak.srce.hr/11427
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-08-07 08:59:12