|Language|| English |
|Title (English)||Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection. |
|Author||Roviš, Tihana Lenac |
Bailer, Susanne M.
Pothineni, Venkata R.
Ouwendijk, Werner J. D.
Verweij, Marieke C.
von Brunn, Albrecht
Verjans, Georges M. G. M.
|Abstract (English)|| |
Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.
|Keywords (English)||Animals Antibodies, Monoclonal/immunology Blotting, Western Cell Line Cell Line Tumor Chickenpox/virology Epithelial Cells/virology Fluorescent Antibody Technique,Indirect Herpes Zoster/virology Herpesvirus 3 Human/immunology Herpesvirus 3, Human/metabolism Humans Mice Mice, Inbred BALB C Proteomics Skin/immunology Skin/virology Viral Proteins/immunology Viral Proteins/metabolism |
|Publication type||scientific paper - original scientific paper |
|Publication status||published |
|Peer review||peer review - international |
|Journal title||Journal of virology |
|Numbering||2013, Vol. 87, No. 12, pp 6943-6954 |
|Date||publication: 2013. |
|DOI identifier||10.1128/JVI.00407-13 |
|Article URL||https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676123/ |
|Scientific field||BIOMEDICINE AND HEALTHCARE |
Basic Medical Sciences
|Institution||University of Rijeka, Faculty of Medicine |
(Center for Proteomics)
|Project||Project number: 229585 |
Project title (English): The Center for Antibody Production Rijeka: Upgrading the Central Research and Service Infrastructure for the South Eastern Region of Europe
Project acronym: CAPRI2010
Project funder: EC
Funding stream: FP7